HPLC-determination of visnagin in genetically transformed root cultures of Ammi visnaga growing in liquid medium

László Kursinszki, Jekaterina Troilina, Éva Szõke

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A combined solid-phase extraction (SPE) high-performance liquid chromatography (HPLC) method was developed for the separation of the furanochromone fraction and for the determination of visnagin in genetically transformed root cultures of Ammi visnaga and their liquid medium. High and reproducible recovery of visnagin was achieved using Supelclean LC-8 SPE columns for isolation. HPLC analysis was performed on an Eurospher C8 Knauer column. The mobile phase was a 29:28:526:417 (v/v/v/v) mixture of acetonitrile, tetrahydrofuran,30 mmol l-1 citric acid (pH 3.0), and methanol. Visnagin was identified by diode-array detection and from matrix assisted laser desorption/ionisation mass spectrometric (MALDI MS) data. The quantification of visnagin was performed by the external standard method. In contrast to the crude drug, the visnagin content was consistently higher than that of khellin in the transformed root cultures.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalChemia Analityczna
Volume45
Issue number2
Publication statusPublished - Dec 1 2000

Keywords

  • HPLC
  • MALDI
  • Root cultures
  • Solid phase extraction
  • Visnagin

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'HPLC-determination of visnagin in genetically transformed root cultures of Ammi visnaga growing in liquid medium'. Together they form a unique fingerprint.

  • Cite this