HPLC determination of K027 in the body of pregnant mice

S. M. Nurulain, S. Ojha, S. Dhanasekaran, K. Kuča, N. Nalin, C. Sharma, A. Adem, H. Kalász

Research output: Contribution to journalArticle

Abstract

Distribution of K027, a hydrophilic, positively charged compound is monitored in the body of pregnant mice using high-performance liquid chromatography (HPLC). Intraperitoneal injection was done on the 18th day of pregnancy; the plasma and brains of the mother mice, placentae and the fetuses' brains were dissected following 5, 15, 30, 60, and 120 min of treatment. Significant incorporation of K027 was found in the placentae and in fetuses' brains relative to its levels in the mothers' plasma and brains. This incorporation warns of a possible adjustment of dose of pyridinium aldoxime antidotes in case of pregnancy. Further studies with different gestational periods and animal models are warranted.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalActa Chromatographica
Volume29
Issue number1
DOIs
Publication statusPublished - Mar 1 2017

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High performance liquid chromatography
Brain
Plasmas
Antidotes
Animals

Keywords

  • Blood-placenta distribution
  • Drug distribution
  • HPLC
  • K027
  • Pregnant mice
  • Pups

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Nurulain, S. M., Ojha, S., Dhanasekaran, S., Kuča, K., Nalin, N., Sharma, C., ... Kalász, H. (2017). HPLC determination of K027 in the body of pregnant mice. Acta Chromatographica, 29(1), 85-93. https://doi.org/10.1556/1326.2017.29.1.06

HPLC determination of K027 in the body of pregnant mice. / Nurulain, S. M.; Ojha, S.; Dhanasekaran, S.; Kuča, K.; Nalin, N.; Sharma, C.; Adem, A.; Kalász, H.

In: Acta Chromatographica, Vol. 29, No. 1, 01.03.2017, p. 85-93.

Research output: Contribution to journalArticle

Nurulain, SM, Ojha, S, Dhanasekaran, S, Kuča, K, Nalin, N, Sharma, C, Adem, A & Kalász, H 2017, 'HPLC determination of K027 in the body of pregnant mice', Acta Chromatographica, vol. 29, no. 1, pp. 85-93. https://doi.org/10.1556/1326.2017.29.1.06
Nurulain SM, Ojha S, Dhanasekaran S, Kuča K, Nalin N, Sharma C et al. HPLC determination of K027 in the body of pregnant mice. Acta Chromatographica. 2017 Mar 1;29(1):85-93. https://doi.org/10.1556/1326.2017.29.1.06
Nurulain, S. M. ; Ojha, S. ; Dhanasekaran, S. ; Kuča, K. ; Nalin, N. ; Sharma, C. ; Adem, A. ; Kalász, H. / HPLC determination of K027 in the body of pregnant mice. In: Acta Chromatographica. 2017 ; Vol. 29, No. 1. pp. 85-93.
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