HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi

Gy Kovács, I. N. Kuzovkina, E. Szőke, L. Kursinszki

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A simple and sensitive liquid chromatographic method has been established for determination of the main flavonoid components of Scutellaria baicalensis Georgi (Lamiaceae) roots and hairy-root cultures. Wogon, the dried root of the plant, is used in traditional Chinese medicine for treatment of bronchitis, hepatitis, tumors, and inflammatory diseases. Lyophilized hairy roots were extracted with methanol. The crude extracts were purified by SPE on Supelco LC-8 cartridges. HPLC separations were performed on a Eurospher 100-C8 reversed-phase column. The mobile phase was a gradient prepared from mixtures of acetonitrile and 0.1% trifluoroacetic acid. Peaks were identified by addition of standards and/or by diode-array detection. Baicalein 7-O-glucuronide (baicalin), wogonin 7-O-glucuronide (wogonoside), baicalein, wogonin, and acteoside were determined by the external standard method at 280 nm. We found that the aglycon (baicalein and wogonin) content of the transformed roots was consistently higher than that of the intact root from Siberia.

Original languageEnglish
JournalChromatographia
Volume60
Issue numberSUPPL.
Publication statusPublished - 2004

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Scutellaria baicalensis
Flavonoids
Glucuronides
High Pressure Liquid Chromatography
Siberia
Lamiaceae
Trifluoroacetic Acid
Plant Roots
Bronchitis
Chinese Traditional Medicine
Complex Mixtures
Hepatitis
Medicine
Methanol
Tumors
Diodes
Liquids
baicalein
wogonin
Neoplasms

Keywords

  • Baicalin, baicalein
  • Column liquid chromatography
  • Hairy root
  • Scutellaria baicalensis
  • Wogonin
  • Wogonoside

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi. / Kovács, Gy; Kuzovkina, I. N.; Szőke, E.; Kursinszki, L.

In: Chromatographia, Vol. 60, No. SUPPL., 2004.

Research output: Contribution to journalArticle

Kovács, Gy ; Kuzovkina, I. N. ; Szőke, E. ; Kursinszki, L. / HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi. In: Chromatographia. 2004 ; Vol. 60, No. SUPPL.
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