HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi

Gy Kovács, I. N. Kuzovkina, É Szoke, L. Kursinszki

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45 Citations (Scopus)

Abstract

A simple and sensitive liquid chromatographic method has been established for determination of the main flavonoid components of Scutellaria baicalensis Georgi (Lamiaceae) roots and hairy-root cultures. Wogon, the dried root of the plant, is used in traditional Chinese medicine for treatment of bronchitis, hepatitis, tumors, and inflammatory diseases. Lyophilized hairy roots were extracted with methanol. The crude extracts were purified by SPE on Supelco LC-8 cartridges. HPLC separations were performed on a Eurospher 100-C8 reversed-phase column. The mobile phase was a gradient prepared from mixtures of acetonitrile and 0.1% trifluoroacetic acid. Peaks were identified by addition of standards and/or by diode-array detection. Baicalein 7-O-glucuronide (baicalin), wogonin 7-O-glucuronide (wogonoside), baicalein, wogonin, and acteoside were determined by the external standard method at 280 nm. We found that the aglycon (baicalein and wogonin) content of the transformed roots was consistently higher than that of the intact root from Siberia.

Original languageEnglish
Pages (from-to)S81-S85
JournalChromatographia
Volume60
Issue numberSUPPL.
Publication statusPublished - Aug 20 2004

Keywords

  • Baicalin, baicalein
  • Column liquid chromatography
  • Hairy root
  • Scutellaria baicalensis
  • Wogonin
  • Wogonoside

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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  • Cite this

    Kovács, G., Kuzovkina, I. N., Szoke, É., & Kursinszki, L. (2004). HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi. Chromatographia, 60(SUPPL.), S81-S85.