An improved RP-HPLC method on ODS-Hypersil column with pre-column derivatization with Marfey's reagent were used to monitor racemization in N-, C-and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic samples were obtained by partial deprotection of different starting materials. In a simple two-step procedure (deprotection and derivatization) the compounds of amino acids formed stable diastereomeric derivatives having facile resolutions.
ASJC Scopus subject areas
- Molecular Medicine