Hplc determination of enantiomeric purity of protected amino acid derivatives used in peptide synthesis

Gy Szókán, Sz Hadfi, K. Krizsán, A. Liembeck, I. Krecz, M. Almás, Cs Somlai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An improved RP-HPLC method on ODS-Hypersil column with pre-column derivatization with Marfey's reagent were used to monitor racemization in N-, C-and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic samples were obtained by partial deprotection of different starting materials. In a simple two-step procedure (deprotection and derivatization) the compounds of amino acids formed stable diastereomeric derivatives having facile resolutions.

Original languageEnglish
Pages (from-to)2759-2775
Number of pages17
JournalJournal of Liquid Chromatography
Volume17
Issue number13
DOIs
Publication statusPublished - Aug 1 1994

ASJC Scopus subject areas

  • Molecular Medicine

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