HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine

H. Kalász, A. Benkö, Z. Szücs, Á Szilágyi, T. Szarvas, J. Lengyel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Administration of 14C-labelled L-deprenyl to rats results in the urinary elimination of a 14C-labelled compound. The 9-fluorenylmethoxycarbonyl chloride-reacted urine sample is fractionated by high-performance liquid chromatography (HPLC) on an octadecyl silica stationary phase. Nε-Monomethyl-lysine is identified in the fraction containing the majority of the radioactivity. Structural elucidation is carried out using HPLC-mass spectrometry in atmospheric pressure chemical ionization mode. Identification of the 14C-labelled fragment in Nε-monomethyl- lysine is an experimental proof that an N-methylated amino acid is generated by transmethylation from a well-known drug. This type of transmethylation may have basic importance in the positive side effects of certain drugs.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalJournal of Chromatographic Science
Volume43
Issue number4
Publication statusPublished - Apr 2005

Fingerprint

High performance liquid chromatography
Lysine
High Pressure Liquid Chromatography
Selegiline
Atmospheric Pressure
Radioactivity
Drug-Related Side Effects and Adverse Reactions
Silicon Dioxide
Pharmaceutical Preparations
Atmospheric pressure
Ionization
Mass spectrometry
Rats
Mass Spectrometry
Urine
Amino Acids
1-(9-fluorenyl)methyl chloroformate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Kalász, H., Benkö, A., Szücs, Z., Szilágyi, Á., Szarvas, T., & Lengyel, J. (2005). HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine. Journal of Chromatographic Science, 43(4), 165-168.

HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine. / Kalász, H.; Benkö, A.; Szücs, Z.; Szilágyi, Á; Szarvas, T.; Lengyel, J.

In: Journal of Chromatographic Science, Vol. 43, No. 4, 04.2005, p. 165-168.

Research output: Contribution to journalArticle

Kalász, H, Benkö, A, Szücs, Z, Szilágyi, Á, Szarvas, T & Lengyel, J 2005, 'HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine', Journal of Chromatographic Science, vol. 43, no. 4, pp. 165-168.
Kalász H, Benkö A, Szücs Z, Szilágyi Á, Szarvas T, Lengyel J. HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine. Journal of Chromatographic Science. 2005 Apr;43(4):165-168.
Kalász, H. ; Benkö, A. ; Szücs, Z. ; Szilágyi, Á ; Szarvas, T. ; Lengyel, J. / HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine. In: Journal of Chromatographic Science. 2005 ; Vol. 43, No. 4. pp. 165-168.
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