HPLC analysis of the flavonoids in pharmaceutical preparations from Canadian goldenrod (Solidago canadensis)

P. Apati, K. Szentmihályi, A. Balázs, D. Baumann, M. Hamburger, T. Sz Kristó, E. Szőke, A. Kéry

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Solidago canadensis L., Canadian goldenrod (Asteraceae) has been used in European phytotheraphy for centuries as a component of urological and antiphlogistical remedies. High-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and online mass spectrometry (MS) has been used for the separation and quantification of phenolics (chlorogenic acid, caffeic acid, kaempferol-3-O-α-L-rutinoside (nicotiflorin), quercetin-3-O-β-D-rutinoside (rutin), quercetin-3-O-β-D-galactoside (hyperoside), quercetin-3-O-β-D-glucoside (isoquercitrin), quercetin-3-O-β-D-rhamnoside (quercitrin), kaempferol-3-O-α-L-rhamnoside (afzelin) and quercetin from Solidaginis herba. Extracts have been obtained using different technologies. Three aqueous and three alcoholic extracts were studied separately. Reversed-phase high-performance liquid chromatography separation of polyphenals on octadecyl sorbent Hypersil was performed, using acetonitrile: acetic acid 2.5 v/v% as eluent in gradient elution. Our results confirm previous reports concerning the presence of several flavonoids. Quantification of the main quercetin glycosides in pharmaceuticals is also reported.

Original languageEnglish
JournalChromatographia
Volume56
Issue numberSUPPL.
Publication statusPublished - 2002

Fingerprint

Solidago
Quercetin
High performance liquid chromatography
Flavonoids
High Pressure Liquid Chromatography
Pharmaceutical Preparations
Chlorogenic Acid
Asteraceae
Glucosides
Reverse-Phase Chromatography
Glycosides
Acetic Acid
Mass Spectrometry
Galactosides
Technology
Sorbents
Mass spectrometry
Diodes
quercitrin
hyperoside

Keywords

  • Column liquid chromatography
  • Flavonol glycosides
  • Identification and quantification
  • Mass spectrometry
  • Solidago canadensis L.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

HPLC analysis of the flavonoids in pharmaceutical preparations from Canadian goldenrod (Solidago canadensis). / Apati, P.; Szentmihályi, K.; Balázs, A.; Baumann, D.; Hamburger, M.; Kristó, T. Sz; Szőke, E.; Kéry, A.

In: Chromatographia, Vol. 56, No. SUPPL., 2002.

Research output: Contribution to journalArticle

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AU - Szentmihályi, K.

AU - Balázs, A.

AU - Baumann, D.

AU - Hamburger, M.

AU - Kristó, T. Sz

AU - Szőke, E.

AU - Kéry, A.

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