HPLC analysis of the flavonoids in pharmaceutical preparations from Canadian goldenrod (Solidago canadensis)

P. Apati, K. Szentmihályi, A. Balázs, D. Baumann, M. Hamburger, T. Sz Kristó, É Szoke, Á Kéry

Research output: Contribution to journalArticle

29 Citations (Scopus)


Solidago canadensis L., Canadian goldenrod (Asteraceae) has been used in European phytotheraphy for centuries as a component of urological and antiphlogistical remedies. High-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and online mass spectrometry (MS) has been used for the separation and quantification of phenolics (chlorogenic acid, caffeic acid, kaempferol-3-O-α-L-rutinoside (nicotiflorin), quercetin-3-O-β-D-rutinoside (rutin), quercetin-3-O-β-D-galactoside (hyperoside), quercetin-3-O-β-D-glucoside (isoquercitrin), quercetin-3-O-β-D-rhamnoside (quercitrin), kaempferol-3-O-α-L-rhamnoside (afzelin) and quercetin from Solidaginis herba. Extracts have been obtained using different technologies. Three aqueous and three alcoholic extracts were studied separately. Reversed-phase high-performance liquid chromatography separation of polyphenals on octadecyl sorbent Hypersil was performed, using acetonitrile: acetic acid 2.5 v/v% as eluent in gradient elution. Our results confirm previous reports concerning the presence of several flavonoids. Quantification of the main quercetin glycosides in pharmaceuticals is also reported.

Original languageEnglish
Pages (from-to)S65-S68
Issue numberSUPPL.
Publication statusPublished - Jan 1 2002


  • Column liquid chromatography
  • Flavonol glycosides
  • Identification and quantification
  • Mass spectrometry
  • Solidago canadensis L.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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