HPLC analysis of in vivo intestinal absorption and oxidative metabolism of salicylic acid in the rat

Mónika Kuzma, Eszter Nyúl, Mátyás Mayer, Emil Fischer, P. Perjési

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In vivo absorption and oxidative metabolism of salicylic acid in rat small intestine was studied by luminal perfusion experiment. Perfusion through the lumen of proximal jejunum with isotonic medium containing 250 μm sodium salicylate was carried out. Absorption of salicylate was measured by a validated HPLC-DAD method which was evaluated for a number of validation characteristics (specificity, repeatability and intermediate precision, limit of detection, limit of quantification, linearity and accuracy). The method was linear over the concentration range 0.5–50 μg/mL. After liquid–liquid extraction of the perfusion samples oxidative biotransformation of salicylate was also investigated by HPLC-MS. The method was linear over the concentration range 0.25–5.0 μg/mL. Two hydroxylated metabolites of salicylic acid (2,5-dihydroxybenzoic acid and 2,3-dihydroxybenzoic acid) were detected and identified. The mean recovery of extraction was 72.4% for 2,3-DHB, 72.5% for 2,5-DHB and 50.1% for salicylic acid, respectively. The methods were successfully applied to investigate jejunal absorption and oxidative metabolism of sodium salicylate in experimental animals. The methods provide analytical background for further metabolic studies of salycilates under modified physiological conditions.

Original languageEnglish
Pages (from-to)2044-2052
Number of pages9
JournalBiomedical Chromatography
Volume30
Issue number12
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Salicylic Acid
Intestinal Absorption
Metabolism
Sodium Salicylate
Rats
Salicylates
High Pressure Liquid Chromatography
Perfusion
Metabolites
Animals
Recovery
Jejunum
Biotransformation
Small Intestine
Limit of Detection
Experiments

Keywords

  • HPLC-MS
  • intestinal metabolism
  • oxidative metabolism
  • salicylic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

HPLC analysis of in vivo intestinal absorption and oxidative metabolism of salicylic acid in the rat. / Kuzma, Mónika; Nyúl, Eszter; Mayer, Mátyás; Fischer, Emil; Perjési, P.

In: Biomedical Chromatography, Vol. 30, No. 12, 01.12.2016, p. 2044-2052.

Research output: Contribution to journalArticle

Kuzma, Mónika ; Nyúl, Eszter ; Mayer, Mátyás ; Fischer, Emil ; Perjési, P. / HPLC analysis of in vivo intestinal absorption and oxidative metabolism of salicylic acid in the rat. In: Biomedical Chromatography. 2016 ; Vol. 30, No. 12. pp. 2044-2052.
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