HPLC analysis of carotenoids from tomatoes using cross-linked C18 column and MS detection

H. Daood, Gyula Bencze, Gábor Palotás, Z. Pék, Akmal Sidikov, L. Helyes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study was conducted to analyze carotenoids from tomatoes by high-performance liquid chromatography using reversed-phase C18 silica having cross-linked end-capping with diode array and mass spectrometric detection. An efficient gradient elution system was developed to achieve good and reliable separation of both major and minor carotenoids as well as their isomers. Resolution of lycopene, β-carotene and their isomers was 0.91-3.97 and 1.02-2.86 with cross-linked and conventional C18 column, respectively. The % recovery for zeaxanthin, lycopene and β-carotene was found to be in the range of 89-97%. Limits of detection and quantification of 19.44 and 64.79 ng/mL for zeaxanthin, 15.6 and 52.4 ng/mL for lycopene and 8.28 and 27.61 ng/mL for β-carotene were determined. More ca-rotenoid compounds could be separated and detected with the new method as compared with conventional C18 column. Hyphenation of HPLC with photodiode array and mass spectrometry detectors assisted in detection of tetra-dehydrocarotenoid and fatty acid diesters of xanthophylls in tomato products. Content of all-trans-lycopene, β-carotene and total carotenoid in different industrial tomatoes tested was found to range between 41.87 and 84.65, 0.89 and 1.50 and 53.22 and 112.60 μg/g fresh weight, respectively.

Original languageEnglish
Pages (from-to)985-991
Number of pages7
JournalJournal of Chromatographic Science
Volume52
Issue number9
DOIs
Publication statusPublished - 2014

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Carotenoids
Lycopersicon esculentum
High Pressure Liquid Chromatography
Isomers
Xanthophylls
Silicon Dioxide
High performance liquid chromatography
Limit of Detection
Photodiodes
Mass Spectrometry
Fatty Acids
Mass spectrometry
Diodes
Weights and Measures
lycopene
Detectors
Recovery

ASJC Scopus subject areas

  • Analytical Chemistry
  • Medicine(all)

Cite this

HPLC analysis of carotenoids from tomatoes using cross-linked C18 column and MS detection. / Daood, H.; Bencze, Gyula; Palotás, Gábor; Pék, Z.; Sidikov, Akmal; Helyes, L.

In: Journal of Chromatographic Science, Vol. 52, No. 9, 2014, p. 985-991.

Research output: Contribution to journalArticle

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