HPLC analysis of blood-brain barrier penetration of 4-fluorodeprenyl

Zita Pöstényi, K. Tekes, Edit Tóth-Molnár, H. Kalász

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30. mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60. min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24. μg/g, showing maximum concentration in the brain and the liver after 5. min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15. min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume102
DOIs
Publication statusPublished - Jan 5 2015

Fingerprint

Blood-Brain Barrier
Lacrimal Apparatus
High Pressure Liquid Chromatography
Liver
Rats
Testis
Brain
Serum
Calibration
Wistar Rats
Tissue
4-fluorodeprenyl

Keywords

  • (-)-4-Fluorodeprenyl
  • Blood-Brain barrier (BBB)
  • HPLC
  • Lacrimal gland
  • Rats

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry
  • Medicine(all)

Cite this

HPLC analysis of blood-brain barrier penetration of 4-fluorodeprenyl. / Pöstényi, Zita; Tekes, K.; Tóth-Molnár, Edit; Kalász, H.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 102, 05.01.2015, p. 529-534.

Research output: Contribution to journalArticle

@article{d7baea30fdaa4d9ab03b9b7941415743,
title = "HPLC analysis of blood-brain barrier penetration of 4-fluorodeprenyl",
abstract = "Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30. mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60. min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24. μg/g, showing maximum concentration in the brain and the liver after 5. min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15. min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.",
keywords = "(-)-4-Fluorodeprenyl, Blood-Brain barrier (BBB), HPLC, Lacrimal gland, Rats",
author = "Zita P{\"o}st{\'e}nyi and K. Tekes and Edit T{\'o}th-Moln{\'a}r and H. Kal{\'a}sz",
year = "2015",
month = "1",
day = "5",
doi = "10.1016/j.jpba.2014.09.035",
language = "English",
volume = "102",
pages = "529--534",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

TY - JOUR

T1 - HPLC analysis of blood-brain barrier penetration of 4-fluorodeprenyl

AU - Pöstényi, Zita

AU - Tekes, K.

AU - Tóth-Molnár, Edit

AU - Kalász, H.

PY - 2015/1/5

Y1 - 2015/1/5

N2 - Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30. mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60. min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24. μg/g, showing maximum concentration in the brain and the liver after 5. min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15. min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.

AB - Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30. mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60. min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24. μg/g, showing maximum concentration in the brain and the liver after 5. min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15. min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.

KW - (-)-4-Fluorodeprenyl

KW - Blood-Brain barrier (BBB)

KW - HPLC

KW - Lacrimal gland

KW - Rats

UR - http://www.scopus.com/inward/record.url?scp=84909606122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84909606122&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2014.09.035

DO - 10.1016/j.jpba.2014.09.035

M3 - Article

C2 - 25459953

AN - SCOPUS:84909606122

VL - 102

SP - 529

EP - 534

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -