HPLC analysis of anthraquinone derivatives in madder root (Rubia tinctorum) and its cell cultures

K. Krizsán, Gy Szókán, Z. A. Tóth, F. Hollósy, M. László, A. Khlafulla

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A sensitive and reproducible RP-HPLC method was developed for the characterization of madder root and its cell cultures extracts and for the determination of anthraquinone derivatives as glycosides (ruberythric acid, lucidin primveroside) and aglycones (alizarin, lucidin, purpurin) in them. The fingerprint chromatographic patterns of the extracts were obtained on C18 silica column, using gradient with acetonitrile-water-acidic buffer eluent system. For quantitative measurements, the anthraquinone derivatives were separated by isocratic elution in the same type of mobile phase. The chief components were identified and quantitatively determined. The natural plant and its cell suspension cultures were compared to each other. The preparative fractionation of the extracts was achieved by gel chromatography, HPLC and selective extractions with solvent series, solid-phase extraction techniques and SFE.

Original languageEnglish
Pages (from-to)2295-2314
Number of pages20
JournalJournal of Liquid Chromatography and Related Technologies
Volume19
Issue number14
Publication statusPublished - 1996

Fingerprint

Rubia
Anthraquinones
Cell culture
Cell Culture Techniques
High Pressure Liquid Chromatography
Solid Phase Extraction
Plant Cells
Dermatoglyphics
Fractionation
Glycosides
Chromatography
Cell Extracts
Silicon Dioxide
Gel Chromatography
Suspensions
Buffers
Gels
Water
lucidin

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

HPLC analysis of anthraquinone derivatives in madder root (Rubia tinctorum) and its cell cultures. / Krizsán, K.; Szókán, Gy; Tóth, Z. A.; Hollósy, F.; László, M.; Khlafulla, A.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 19, No. 14, 1996, p. 2295-2314.

Research output: Contribution to journalArticle

Krizsán, K. ; Szókán, Gy ; Tóth, Z. A. ; Hollósy, F. ; László, M. ; Khlafulla, A. / HPLC analysis of anthraquinone derivatives in madder root (Rubia tinctorum) and its cell cultures. In: Journal of Liquid Chromatography and Related Technologies. 1996 ; Vol. 19, No. 14. pp. 2295-2314.
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