HPCE analysis of hydrolysing morphine derivatives. Quantitation of decomposition rate and mobility

D. Visky, M. Kraszni, S. Hosztafi, B. Noszál

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

High performance capillary electrophoretic conditions were optimised on the basis of protonation constants and hydrolysis rate constants (if relevant) of 6 opiate compounds. Protonation constants were determined by pH- potentiometry, the progress of hydrolysis was followed by capillary electrophoresis, hydrolysis rate constants of ester-type compounds were elucidated by kinetic studies of the time- and pH-dependence of the decomposition. Using these physico-chemical parameters, the analysis circumstances were designed to keep the in situ hydrolysis rate negligible for every compound, which has not been the case in reported previous HPCE determinations of acetylated derivatives. Our calculated charge-related mobility differences and experimental CZE findings justified those earlier statements that capillary zone electrophoresis is insufficient to separate these compounds. The method development for diacetylmorphine, 3- acetylmorphine, 6-acetylmorphine, morphine, acetylcodeine and codeine resulted in the use of a micellar electrokinetic system operating at pH = 8.0, applying 50 mM sodium dodecyl sulfate micelle-forming agent and 7.5% acetonitrile additive in the background electrolyte.

Original languageEnglish
Pages (from-to)294-300
Number of pages7
JournalChromatographia
Volume51
Issue number5-6
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Capillary electrophoresis
  • Heroin
  • Morphine
  • Rate of hydrolysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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