How to investigate interactions between membrane proteins and ligands by solid-state NMR

Andrea Lakatos, Karsten Mörs, Clemens Glaubitz

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Solid-state NMR is an established method for biophysical studies of membrane proteins within the lipid bilayers and an emerging technique for structural biology in general. In particular magic angle sample spinning has been found to be very useful for the investigation of large membrane proteins and their interaction with small molecules within the lipid bilayer. Using a number of examples, we illustrate and discuss in this chapter, which information can be gained and which experimental parameters need to be considered when planning such experiments. We focus especially on the interaction of diffusive ligands with membrane proteins.

Original languageEnglish
Title of host publicationMembrane Protein Structure and Dynamics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages65-86
Number of pages22
ISBN (Print)9781627030229
DOIs
Publication statusPublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume914
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Membrane proteins
  • Protein-ligand interactions
  • Solid-state NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lakatos, A., Mörs, K., & Glaubitz, C. (2012). How to investigate interactions between membrane proteins and ligands by solid-state NMR. In Membrane Protein Structure and Dynamics: Methods and Protocols (pp. 65-86). (Methods in Molecular Biology; Vol. 914). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-23-6_5