How to determine the efficiency of intermediate transfer in an interacting enzyme system?

Péter Tompa, József Batke, Judit Ovádi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A kinetic method, based upon measuring the transient time of coupled reactions, is proposed for the determination of the intermediate channel efficiency in a system of functionally interacting enzymes. The procedure rests upon a novel description in which the transient time is expressed as a function of channel efficiency and lifetime of the intermediate molecules. By this approach the reduction of transient time can be explained even if no changes in the kinetic parameters of the individual reactions occur. For determining channel efficiency, a linearized form has been evaluated and applied to the analysis of the kinetics of the aspartate aminotransferase-glutamate dehydrogenase coupled reaction, for which the data were taken from the literature [(1982) Eur. J. Biochem. 121, 511-517].

Original languageEnglish
Pages (from-to)244-248
Number of pages5
JournalFEBS letters
Volume214
Issue number2
DOIs
Publication statusPublished - Apr 20 1987

Keywords

  • Channeling
  • Enzyme interaction
  • Intermediate transfer efficiency

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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