How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'

J. Dobránszki, I. Hudák, K. Magyar-Tábori, Z. Galli, E. Kiss, E. Jámbor-Benczúr

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron (TDZ), or benzyladenine (BA), or meta-topolin (TOP), or zeatin (ZEA), or kinetin (KIN), or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg L-1. Effects of these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN) and organogenetic index (OI) calculated from these data. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, OI: 7.5) was achieved in 'Royal Gala' by using 0.5 mg L-1 TDZ. There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins. In 'M.26' SN was the highest (3.22) using 6.5 mg L-1 BAR or 8.0 mg L-1 TOPR (3.18). SN was not significantly lower (3.12) by using 2.0 mg L -1 TDZ; however, OI was about half as big (0.63 compared to 1.29 or 1.74 with BAR or TOPR, respectively). 'Royal Gala' had higher organogenetic ability, than 'M.26': 3.5-fold higher SN and more than 4-fold higher OI was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26'.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages191-196
Number of pages6
Volume725 I
Publication statusPublished - Nov 2006

Publication series

NameActa Horticulturae
Volume725 I
ISSN (Print)05677572

Fingerprint

cytokinins
apples
shoots
leaves
explants
vitrification
zeatin
scions
organogenesis
kinetin
chemical structure
benzyladenine
rootstocks
developmental stages
genotype
cultivars
thidiazuron

Keywords

  • Malus x domestica
  • Organogenetic ability
  • Time course of regeneration
  • Type of cytokinins

ASJC Scopus subject areas

  • Horticulture

Cite this

Dobránszki, J., Hudák, I., Magyar-Tábori, K., Galli, Z., Kiss, E., & Jámbor-Benczúr, E. (2006). How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'. In Acta Horticulturae (Vol. 725 I, pp. 191-196). (Acta Horticulturae; Vol. 725 I).

How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'. / Dobránszki, J.; Hudák, I.; Magyar-Tábori, K.; Galli, Z.; Kiss, E.; Jámbor-Benczúr, E.

Acta Horticulturae. Vol. 725 I 2006. p. 191-196 (Acta Horticulturae; Vol. 725 I).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dobránszki, J, Hudák, I, Magyar-Tábori, K, Galli, Z, Kiss, E & Jámbor-Benczúr, E 2006, How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'. in Acta Horticulturae. vol. 725 I, Acta Horticulturae, vol. 725 I, pp. 191-196.
Dobránszki J, Hudák I, Magyar-Tábori K, Galli Z, Kiss E, Jámbor-Benczúr E. How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'. In Acta Horticulturae. Vol. 725 I. 2006. p. 191-196. (Acta Horticulturae).
Dobránszki, J. ; Hudák, I. ; Magyar-Tábori, K. ; Galli, Z. ; Kiss, E. ; Jámbor-Benczúr, E. / How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'. Acta Horticulturae. Vol. 725 I 2006. pp. 191-196 (Acta Horticulturae).
@inproceedings{d32551ec133749da8551fd62833ed63e,
title = "How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'",
abstract = "The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron (TDZ), or benzyladenine (BA), or meta-topolin (TOP), or zeatin (ZEA), or kinetin (KIN), or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg L-1. Effects of these cytokinins were evaluated on the percentage of regeneration (R{\%}) and that of vitrification (V{\%}) and on the number of regenerated shoots per explant (SN) and organogenetic index (OI) calculated from these data. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, OI: 7.5) was achieved in 'Royal Gala' by using 0.5 mg L-1 TDZ. There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins. In 'M.26' SN was the highest (3.22) using 6.5 mg L-1 BAR or 8.0 mg L-1 TOPR (3.18). SN was not significantly lower (3.12) by using 2.0 mg L -1 TDZ; however, OI was about half as big (0.63 compared to 1.29 or 1.74 with BAR or TOPR, respectively). 'Royal Gala' had higher organogenetic ability, than 'M.26': 3.5-fold higher SN and more than 4-fold higher OI was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26'.",
keywords = "Malus x domestica, Organogenetic ability, Time course of regeneration, Type of cytokinins",
author = "J. Dobr{\'a}nszki and I. Hud{\'a}k and K. Magyar-T{\'a}bori and Z. Galli and E. Kiss and E. J{\'a}mbor-Bencz{\'u}r",
year = "2006",
month = "11",
language = "English",
isbn = "906605719X",
volume = "725 I",
series = "Acta Horticulturae",
pages = "191--196",
booktitle = "Acta Horticulturae",

}

TY - GEN

T1 - How can different cytokinins influence the process of shoot regeneration from apple leaves in 'Royal Gala' and 'M.26'

AU - Dobránszki, J.

AU - Hudák, I.

AU - Magyar-Tábori, K.

AU - Galli, Z.

AU - Kiss, E.

AU - Jámbor-Benczúr, E.

PY - 2006/11

Y1 - 2006/11

N2 - The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron (TDZ), or benzyladenine (BA), or meta-topolin (TOP), or zeatin (ZEA), or kinetin (KIN), or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg L-1. Effects of these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN) and organogenetic index (OI) calculated from these data. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, OI: 7.5) was achieved in 'Royal Gala' by using 0.5 mg L-1 TDZ. There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins. In 'M.26' SN was the highest (3.22) using 6.5 mg L-1 BAR or 8.0 mg L-1 TOPR (3.18). SN was not significantly lower (3.12) by using 2.0 mg L -1 TDZ; however, OI was about half as big (0.63 compared to 1.29 or 1.74 with BAR or TOPR, respectively). 'Royal Gala' had higher organogenetic ability, than 'M.26': 3.5-fold higher SN and more than 4-fold higher OI was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26'.

AB - The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron (TDZ), or benzyladenine (BA), or meta-topolin (TOP), or zeatin (ZEA), or kinetin (KIN), or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg L-1. Effects of these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN) and organogenetic index (OI) calculated from these data. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, OI: 7.5) was achieved in 'Royal Gala' by using 0.5 mg L-1 TDZ. There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins. In 'M.26' SN was the highest (3.22) using 6.5 mg L-1 BAR or 8.0 mg L-1 TOPR (3.18). SN was not significantly lower (3.12) by using 2.0 mg L -1 TDZ; however, OI was about half as big (0.63 compared to 1.29 or 1.74 with BAR or TOPR, respectively). 'Royal Gala' had higher organogenetic ability, than 'M.26': 3.5-fold higher SN and more than 4-fold higher OI was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26'.

KW - Malus x domestica

KW - Organogenetic ability

KW - Time course of regeneration

KW - Type of cytokinins

UR - http://www.scopus.com/inward/record.url?scp=33846479991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846479991&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:33846479991

SN - 906605719X

SN - 9789066057197

VL - 725 I

T3 - Acta Horticulturae

SP - 191

EP - 196

BT - Acta Horticulturae

ER -