Host metabolite producing endophytic fungi isolated from Hypericum perforatum

Aruna Vigneshwari, Dávid Rakk, Anikó Németh, S. Kocsubé, Noémi Kiss, Dezső Csupor, T. Papp, Biljana Škrbić, C. Vágvölgyi, A. Szekeres

Research output: Contribution to journalArticle

Abstract

In the present study, endophytic fungi have been isolated from various parts of the medicinal herb Hypericum perforatum (St. John’s Wort), which is known as a source of medically important metabolites. The isolated strains were cultured in liquid media and their ability to synthesize hypericin, the secondary metabolite of the host and its suspected precursor, emodin was tested analyzing the extracts of the fermentation broth and the mycelia. The HPLC-UV analysis of the chloroform/methanol extracts of the mycelia revealed that three isolates were able to produce emodin (SZMC 23771, 19.9 ng/mg; SZMC 23772, 20.8 ng/ mg; SZMC 23769, 427.9 ng/mg) and one of them also could synthesize hypericin (SZMC 23769, 320.4 ng/mg). These results were also confirmed via UHPLC-HRMS technique both in full scan and MS/MS mode. The strains producing only emodin belong to the section Alternata of the genus Alternaria, while the isolate producing both metabolites was identified as Epicoccum nigrum. The mycelial extracts of E. nigrum and the Alternaria sp. SZMC 23772 showed higher inhibitory activities in the antimicrobial tests against the six selected bacteria compared to the hypericin and emodin standards in the applied concentration (100 μg/mL), while in case of the Alternaria sp. SZMC 23771 lower inhibition activities were observed on Staphylococcus aureus and Streptomyces albus than the pure compounds.

Original languageEnglish
Article numbere0217060
JournalPloS one
Volume14
Issue number5
DOIs
Publication statusPublished - May 1 2019

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Emodin
emodin
Hypericum
Hypericum perforatum
Metabolites
hypericin
Fungi
Alternaria
Epicoccum nigrum
metabolites
fungi
Mycelium
mycelium
Streptomyces albus
extracts
Streptomyces
Medicinal Plants
Chloroform
chloroform
Fermentation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Host metabolite producing endophytic fungi isolated from Hypericum perforatum. / Vigneshwari, Aruna; Rakk, Dávid; Németh, Anikó; Kocsubé, S.; Kiss, Noémi; Csupor, Dezső; Papp, T.; Škrbić, Biljana; Vágvölgyi, C.; Szekeres, A.

In: PloS one, Vol. 14, No. 5, e0217060, 01.05.2019.

Research output: Contribution to journalArticle

Vigneshwari, Aruna ; Rakk, Dávid ; Németh, Anikó ; Kocsubé, S. ; Kiss, Noémi ; Csupor, Dezső ; Papp, T. ; Škrbić, Biljana ; Vágvölgyi, C. ; Szekeres, A. / Host metabolite producing endophytic fungi isolated from Hypericum perforatum. In: PloS one. 2019 ; Vol. 14, No. 5.
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