Host-cell-phenotype-dependent control of the BCR2/BWR1 promoter complex regulates the expression of Epstein-Barr virus nuclear antigens 2-6

Ender Altiok, J. Mináróvits, Hu Li-Fu, Bertha Contreras-Brodin, George Klein, Ingemar Ernberg

Research output: Contribution to journalArticle

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Abstract

Epstein-Barr virus nuclear antigens (EBNAs) are expressed in a cell-phenotype-dependent manner. EBNA 1 is regularly expressed in all Epstein-Barr virus-carrying cells, whereas EBNAs 2-6 are only expressed in Epstein-Barr virus-carrying cells with a lymphoblastoid phenotype including group III Burkitt lymphoma (BL) lines positive for B-cell activation markers. Transcripts are initiated at the BCR2 or exceptionally at one BWR1 promoter in lymphoblastoid cell lines and group III BL lines. In group I BL lines, nasopharyngeal carcinoma, and the somatic cell hybrids, where EBNAs 2-6 are downregulated, the BCR2/BWR1 promoter complex is inactive or switched off. Upregulation of EBNAs 2-6 in group III BL cells and in 5-azacytidine-treated group I BL cells accompanies the activation of the silent BCR2/BWR1 promoters. Activation of BCR2 parallels demethylation of at least one CpG pair in the same promoter region. The activity of BCR2/BWR1 promoter complex depends on a particular B-cell phenotype. EBNA 1 transcription must be initiated at another promoter in cells that express only EBNA 1.

Original languageEnglish
Pages (from-to)905-909
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number3
Publication statusPublished - Feb 1 1992

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Epstein-Barr Virus Nuclear Antigens
Burkitt Lymphoma
Phenotype
Human Herpesvirus 4
B-Lymphocytes
Azacitidine
Hybrid Cells
Genetic Promoter Regions
Up-Regulation
Down-Regulation
Cell Line

Keywords

  • Burkitt lymphoma
  • Nasopharyngeal carcinoma
  • Promoter usage
  • Somatic hybrid

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Host-cell-phenotype-dependent control of the BCR2/BWR1 promoter complex regulates the expression of Epstein-Barr virus nuclear antigens 2-6. / Altiok, Ender; Mináróvits, J.; Li-Fu, Hu; Contreras-Brodin, Bertha; Klein, George; Ernberg, Ingemar.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 3, 01.02.1992, p. 905-909.

Research output: Contribution to journalArticle

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