Host cell macromolecular synthesis in cells containing EBV-induced early antigens, studied by combined immunofluorescence and radioautography

L. Gergely, George Klein, Ingemar Ernberg

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Host cell macromolecular synthesis was studied in EBV-infected Raji cells, containing the intranuclear early antigens (EA), by combined immunofluorescence and autoradiography. DNA, RNA, and protein synthesis was inhibited in EA+ cells. Membrane antigen (MA) positive, EA negative cells continued an apparently uninfluenced RNA and protein synthesis. The proportion of DNA synthesizing MA+EA- cells was lower than among antigen negative (MA-EA-) cells, but this may be related to the reduced expression of membrane antigens during and after the S phase.

Original languageEnglish
JournalVirology
Volume45
Issue number1
DOIs
Publication statusPublished - 1971

Fingerprint

Autoradiography
Human Herpesvirus 4
Fluorescent Antibody Technique
Antigens
Membranes
RNA
DNA
S Phase
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Host cell macromolecular synthesis in cells containing EBV-induced early antigens, studied by combined immunofluorescence and radioautography. / Gergely, L.; Klein, George; Ernberg, Ingemar.

In: Virology, Vol. 45, No. 1, 1971.

Research output: Contribution to journalArticle

@article{b4f10128b3184fa1bc6bb178c3fa0c41,
title = "Host cell macromolecular synthesis in cells containing EBV-induced early antigens, studied by combined immunofluorescence and radioautography",
abstract = "Host cell macromolecular synthesis was studied in EBV-infected Raji cells, containing the intranuclear early antigens (EA), by combined immunofluorescence and autoradiography. DNA, RNA, and protein synthesis was inhibited in EA+ cells. Membrane antigen (MA) positive, EA negative cells continued an apparently uninfluenced RNA and protein synthesis. The proportion of DNA synthesizing MA+EA- cells was lower than among antigen negative (MA-EA-) cells, but this may be related to the reduced expression of membrane antigens during and after the S phase.",
author = "L. Gergely and George Klein and Ingemar Ernberg",
year = "1971",
doi = "10.1016/0042-6822(71)90108-5",
language = "English",
volume = "45",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Host cell macromolecular synthesis in cells containing EBV-induced early antigens, studied by combined immunofluorescence and radioautography

AU - Gergely, L.

AU - Klein, George

AU - Ernberg, Ingemar

PY - 1971

Y1 - 1971

N2 - Host cell macromolecular synthesis was studied in EBV-infected Raji cells, containing the intranuclear early antigens (EA), by combined immunofluorescence and autoradiography. DNA, RNA, and protein synthesis was inhibited in EA+ cells. Membrane antigen (MA) positive, EA negative cells continued an apparently uninfluenced RNA and protein synthesis. The proportion of DNA synthesizing MA+EA- cells was lower than among antigen negative (MA-EA-) cells, but this may be related to the reduced expression of membrane antigens during and after the S phase.

AB - Host cell macromolecular synthesis was studied in EBV-infected Raji cells, containing the intranuclear early antigens (EA), by combined immunofluorescence and autoradiography. DNA, RNA, and protein synthesis was inhibited in EA+ cells. Membrane antigen (MA) positive, EA negative cells continued an apparently uninfluenced RNA and protein synthesis. The proportion of DNA synthesizing MA+EA- cells was lower than among antigen negative (MA-EA-) cells, but this may be related to the reduced expression of membrane antigens during and after the S phase.

UR - http://www.scopus.com/inward/record.url?scp=0015096015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015096015&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(71)90108-5

DO - 10.1016/0042-6822(71)90108-5

M3 - Article

VL - 45

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -