Hormones in the nucleus of mast cells

Confocal microscopic immunocytochemical observations

G. Csaba, P. Kovács

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In earlier experiments, the presence of histamine and serotonin in the nucleus of mast cells was demonstrated. At present we studied the presence or absence of four hormones: adrenocorticotropine (ACTH), growth hormone (GH), triiodothyronine (T3), and progesterone. Cells of the (adult female) rat peritoneal fluid were fixed by EDAC and studied by laser-scanning confocal microscope, after treatment with primary antibodies and FITC-labeled secondary antibody. Strong ACTH and growth hormone fluorescence can be seen in the nucleus. ACTH fluorescence is present also in the granules. The cytoplasm of lymphocytes contains both ACTH and GH. T3 shows very pale fluorescence, and progesterone is negative. The conclusion is that the nucleus of mast cells contains two polypeptide hormones studied. This was demonstrated by using EDAC fixation as this is a cross-linking agent and does not allow the escape of the minute amounts of hormones from the nucleus. Earlier observations on peptide hormones nuclear presence and their importance are discussed.

Original languageEnglish
Pages (from-to)621-625
Number of pages5
JournalHormone and Metabolic Research
Volume41
Issue number8
DOIs
Publication statusPublished - 2009

Fingerprint

Mast Cells
Adrenocorticotropic Hormone
Hormones
Growth Hormone
Peptide Hormones
Fluorescence
Progesterone
Lymphocytes
Antibodies
Fluorescein-5-isothiocyanate
Ascitic Fluid
Triiodothyronine
Histamine
Rats
Serotonin
Cytoplasm
Lasers
Microscopes
Scanning
Fluids

Keywords

  • ACTH
  • EDAC fixation
  • GH
  • Mast cells
  • Nuclear hormones
  • Polypeptide hormones

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Medicine(all)

Cite this

Hormones in the nucleus of mast cells : Confocal microscopic immunocytochemical observations. / Csaba, G.; Kovács, P.

In: Hormone and Metabolic Research, Vol. 41, No. 8, 2009, p. 621-625.

Research output: Contribution to journalArticle

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