Hormonal regulation of complement biosynthesis in human cell lines-II. Upregulation of the biosynthesis of complement components C3, factor B and Cl inhibitor by interleukin-6 and interleukin-1 in human hepatoma cell line

A. Falus, H. Rokita, E. Walcz, M. Brozik, T. Hidvégi, K. Merétey

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Abstract

The effect of interleukin (IL)-6 and IL-1 on the biosynthesis of complement components C3, factor B, C2, C4 and Cl inhibitor (Cl inh), as well as that of albumin, was studied in vitro in human hepatoma-derived cell line, HepG2. Measuring the amounts of secreted complement proteins we detected a significant upregulation of C3 by both hormones. The enhancement of the factor B and especially that of Cl inh production was predominant by IL-6. In our experimental system neither IL-1 nor IL-6 affected the biosynthesis of C2 and C4. Albumin secretion was significantly decreased only in the simultaneous presence of IL-1 and IL-6. Detection of the changes in the amounts of C3- and factor B-specific mRNA of HepG2 cells suggests a pretranslational regulation by these cytokines. The secretion of C3 and factor B was markedly potentiated when IL-1 and IL-6 were added together. However only the gene expression of factor B, but not of C3, was found to reveal synergism. IL-6 enhanced the in vitro production of C3 in mouse hepatocytes as well. This effect was greatly potentiated in the presence of histamine.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalMolecular Immunology
Volume27
Issue number2
DOIs
Publication statusPublished - 1990

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Complement C3
Complement Factor B
Interleukin-1
Hepatocellular Carcinoma
Interleukin-6
Up-Regulation
Cell Line
Albumins
Hep G2 Cells
Histamine
Hepatocytes
Complement System Proteins
Hormones
Cytokines
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

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title = "Hormonal regulation of complement biosynthesis in human cell lines-II. Upregulation of the biosynthesis of complement components C3, factor B and Cl inhibitor by interleukin-6 and interleukin-1 in human hepatoma cell line",
abstract = "The effect of interleukin (IL)-6 and IL-1 on the biosynthesis of complement components C3, factor B, C2, C4 and Cl inhibitor (Cl inh), as well as that of albumin, was studied in vitro in human hepatoma-derived cell line, HepG2. Measuring the amounts of secreted complement proteins we detected a significant upregulation of C3 by both hormones. The enhancement of the factor B and especially that of Cl inh production was predominant by IL-6. In our experimental system neither IL-1 nor IL-6 affected the biosynthesis of C2 and C4. Albumin secretion was significantly decreased only in the simultaneous presence of IL-1 and IL-6. Detection of the changes in the amounts of C3- and factor B-specific mRNA of HepG2 cells suggests a pretranslational regulation by these cytokines. The secretion of C3 and factor B was markedly potentiated when IL-1 and IL-6 were added together. However only the gene expression of factor B, but not of C3, was found to reveal synergism. IL-6 enhanced the in vitro production of C3 in mouse hepatocytes as well. This effect was greatly potentiated in the presence of histamine.",
author = "A. Falus and H. Rokita and E. Walcz and M. Brozik and T. Hidv{\'e}gi and K. Mer{\'e}tey",
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T1 - Hormonal regulation of complement biosynthesis in human cell lines-II. Upregulation of the biosynthesis of complement components C3, factor B and Cl inhibitor by interleukin-6 and interleukin-1 in human hepatoma cell line

AU - Falus, A.

AU - Rokita, H.

AU - Walcz, E.

AU - Brozik, M.

AU - Hidvégi, T.

AU - Merétey, K.

PY - 1990

Y1 - 1990

N2 - The effect of interleukin (IL)-6 and IL-1 on the biosynthesis of complement components C3, factor B, C2, C4 and Cl inhibitor (Cl inh), as well as that of albumin, was studied in vitro in human hepatoma-derived cell line, HepG2. Measuring the amounts of secreted complement proteins we detected a significant upregulation of C3 by both hormones. The enhancement of the factor B and especially that of Cl inh production was predominant by IL-6. In our experimental system neither IL-1 nor IL-6 affected the biosynthesis of C2 and C4. Albumin secretion was significantly decreased only in the simultaneous presence of IL-1 and IL-6. Detection of the changes in the amounts of C3- and factor B-specific mRNA of HepG2 cells suggests a pretranslational regulation by these cytokines. The secretion of C3 and factor B was markedly potentiated when IL-1 and IL-6 were added together. However only the gene expression of factor B, but not of C3, was found to reveal synergism. IL-6 enhanced the in vitro production of C3 in mouse hepatocytes as well. This effect was greatly potentiated in the presence of histamine.

AB - The effect of interleukin (IL)-6 and IL-1 on the biosynthesis of complement components C3, factor B, C2, C4 and Cl inhibitor (Cl inh), as well as that of albumin, was studied in vitro in human hepatoma-derived cell line, HepG2. Measuring the amounts of secreted complement proteins we detected a significant upregulation of C3 by both hormones. The enhancement of the factor B and especially that of Cl inh production was predominant by IL-6. In our experimental system neither IL-1 nor IL-6 affected the biosynthesis of C2 and C4. Albumin secretion was significantly decreased only in the simultaneous presence of IL-1 and IL-6. Detection of the changes in the amounts of C3- and factor B-specific mRNA of HepG2 cells suggests a pretranslational regulation by these cytokines. The secretion of C3 and factor B was markedly potentiated when IL-1 and IL-6 were added together. However only the gene expression of factor B, but not of C3, was found to reveal synergism. IL-6 enhanced the in vitro production of C3 in mouse hepatocytes as well. This effect was greatly potentiated in the presence of histamine.

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