HorkaD, a chromosome instability-causing mutation in drosophila, is a dominant-negative allele of lodestar

Tamas Szalontai, Imre Gaspar, Istvan Belecz, Iren Kerekes, Miklos Erdelyi, Imre Boros, Janos Szabad

Research output: Contribution to journalArticle

4 Citations (Scopus)


Correct segregation of chromosomes is particularly challenging during the rapid nuclear divisions of early embryogenesis. This process is disrupted by HorkaD, a dominant-negative mutation in Drosophila melanogaster that causes female sterility due to chromosome tangling and nondisjunction during oogenesis and early embryogenesis. HorkaD also renders chromosomes unstable during spermatogenesis, which leads to the formation of diplo//haplo mosaics, including the gynandromorphs. Complete loss of gene function brings about maternal-effect lethality: embryos of the females without the Horka D-identified gene perish due to disrupted centrosome function, defective spindle assembly, formation of chromatin bridges, and abnormal chromosome segregation during the cleavage divisions. These defects are indicators of mitotic catastrophe and suggest that the gene product acts during the meiotic and the cleavage divisions, an idea that is supported by the observation that germ-line chimeras exhibit excessive germ-line and cleavage function. The gene affected by the HorkaD mutation is lodestar, a member of the helicase-related genes. The HorkaD mutation results in replacement of Ala777 with Thr, which we suggest causes chromosome instability by increasing the affinity of Lodestar for chromatin.

Original languageEnglish
Pages (from-to)367-377
Number of pages11
Issue number2
Publication statusPublished - Feb 2009

ASJC Scopus subject areas

  • Genetics

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