Horizontal transfer of DNA by the uptake of apoptotic bodies

Lars Holmgren, Anna Szeles, E. Rajnavolgyi, Judah Folkman, Georg Klein, Ingemar Ernberg, Kerstin I. Falk

Research output: Contribution to journalArticle

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Abstract

In this study we have raised the question of whether DNA can be transferred from one cell to another by phagocytosis of apoptotic bodies. We have used integrated copies of the Epstein-Barr virus (EBV) as a marker to follow the fate and expression pattern of apoptotic DNA in the phagocytotic host. Apoptosis was induced in EBV-carrying cell lines by irradiation before cultivation with either human fibroblasts, macrophages, or bovine aortic endothelial cells. Analysis of the expression pattern of EBV-encoded genes was performed by immunofluorescent staining as well as in situ hybridization. Cocultivation of apoptotic bodies from lymphoid cell lines containing integrated but not episomal copies of EBV resulted in expression of the EBV- encoded genes EBER and EBNA1 in the recipient cells at a high frequency. Fluorescence in situ hybridization analysis showed uptake of human chromatin as well as integrated EBV-DNA into the nuclei of bovine aortic endothelial cells. These data show that DNA may be rescued and reused from apoptotic bodies by somatic cells. In addition, our findings suggest that apoptotic bodies derived from EBV-carrying B lymphocytes may serve as the source of viral transfer to cells that lack receptors for the EBV virus in vivo.

Original languageEnglish
Pages (from-to)3956-3963
Number of pages8
JournalBlood
Volume93
Issue number11
Publication statusPublished - Jun 1 1999

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Human Herpesvirus 4
Viruses
DNA
Endothelial cells
Endothelial Cells
Complement 3d Receptors
Genes
Cells
Cell Line
Coculture Techniques
Lymphocytes
Extracellular Vesicles
Macrophages
Fluorescence In Situ Hybridization
Phagocytosis
Fibroblasts
Chromatin
In Situ Hybridization
B-Lymphocytes
Apoptosis

ASJC Scopus subject areas

  • Hematology

Cite this

Holmgren, L., Szeles, A., Rajnavolgyi, E., Folkman, J., Klein, G., Ernberg, I., & Falk, K. I. (1999). Horizontal transfer of DNA by the uptake of apoptotic bodies. Blood, 93(11), 3956-3963.

Horizontal transfer of DNA by the uptake of apoptotic bodies. / Holmgren, Lars; Szeles, Anna; Rajnavolgyi, E.; Folkman, Judah; Klein, Georg; Ernberg, Ingemar; Falk, Kerstin I.

In: Blood, Vol. 93, No. 11, 01.06.1999, p. 3956-3963.

Research output: Contribution to journalArticle

Holmgren, L, Szeles, A, Rajnavolgyi, E, Folkman, J, Klein, G, Ernberg, I & Falk, KI 1999, 'Horizontal transfer of DNA by the uptake of apoptotic bodies', Blood, vol. 93, no. 11, pp. 3956-3963.
Holmgren L, Szeles A, Rajnavolgyi E, Folkman J, Klein G, Ernberg I et al. Horizontal transfer of DNA by the uptake of apoptotic bodies. Blood. 1999 Jun 1;93(11):3956-3963.
Holmgren, Lars ; Szeles, Anna ; Rajnavolgyi, E. ; Folkman, Judah ; Klein, Georg ; Ernberg, Ingemar ; Falk, Kerstin I. / Horizontal transfer of DNA by the uptake of apoptotic bodies. In: Blood. 1999 ; Vol. 93, No. 11. pp. 3956-3963.
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