Homologous transformation of Trichoderma hamatum with an endochitinase encoding gene, resulting in increased levels of chitinase activity

Gábor Giczey, Zoltán Kerényi, Géza Dallmann, László Hornok

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A 42-kDa endochitinase encoding gene, Tham-ch, was cloned by screening the genomic library of Trichoderma hamatum strain Tam-61 with a PCR-amplified chitinase sequence from the same fungus. Tham-ch with its own regulatory sequences was reintroduced into the host strain. The integration of the transforming construct was stable only in one copy. Homologous integration occurred in nine transformants, while non-homologous integration was detected in one transformant. All but one transformant expressed higher levels of chitinase activity in comparison to the wild-type recipient strain; the maximum level of increase was 5-fold. Duplicating the copy number of the highly conserved ~42-kDa endochitinase encoding gene appears to be one potential means by which the biocontrol capability of the Trichoderma species might be improved. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalFEMS microbiology letters
Volume165
Issue number2
DOIs
Publication statusPublished - Aug 15 1998

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Keywords

  • Biocontrol
  • Chitinase activity
  • Trichoderma

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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