Homogeneous assay for evaluation of aptamer-protein interaction

Gergely Lautner, Zsófia Balogh, Anna Gyurkovics, Róbert E. Gyurcsányi, Tamás Mészáros

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the KD value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.

Original languageEnglish
Pages (from-to)3929-3931
Number of pages3
JournalAnalyst
Volume137
Issue number17
DOIs
Publication statusPublished - Sep 7 2012

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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