Hofmeister ions control protein dynamics

B. Szalontai, Gergely Nagy, Sashka Krumova, E. Fodor, T. Páli, Stefka G. Taneva, G. Garab, Judith Peters, A. Dér

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background Recently, we have elaborated a thermodynamic theory that could coherently interpret the diverse effects of Hofmeister ions on proteins, based on a single physical parameter, the protein-water interfacial tension (Dér et al., Journal of Physical Chemistry B. 2007, 111, 5344-5350). This theory, implying a "liquid drop model", predicts changes in protein conformational fluctuations upon addition of Hofmeister salts (containing either kosmotropic or chaotropic anions) to the medium. Methods Here, we report experimental tests of this prediction using a complex approach by applying methods especially suited for the detection of protein fluctuation changes (neutron scattering, micro-calorimetry, and Fourier-transform infrared spectroscopy). Results It is demonstrated that Hofmeister salts, via setting the hydrophobic/hydrophilic properties of the protein-water interface, control conformational fluctuations even in the interior of the typical membrane transport protein bacteriorhodopsin, around its temperature-induced, unusual α(II) → α(I) conformational transition between 60 and 90 C. We found that below this transition kosmotropic (COOCH3-), while above it chaotropic (ClO4-) anions increase structural fluctuations of bR. It was also shown that, in each case, an onset of enhanced equilibrium fluctuations presages this phase transition in the course of the thermotropic response of bR. Conclusions These results are in full agreement with the theory, and demonstrate that predictions based on protein-water interfacial tension changes can describe Hofmeister effects and interpret protein dynamics phenomena even in unusual cases. General significance This approach is expected to provide a useful guide to understand the principles governing the interplay between protein interfacial properties and conformational dynamics, in general.

Original languageEnglish
Pages (from-to)4564-4572
Number of pages9
JournalBBA - General Subjects
Volume1830
Issue number10
DOIs
Publication statusPublished - 2013

Fingerprint

Ions
Proteins
Surface Tension
Anions
Surface tension
Water
Salts
Physical Chemistry
Bacteriorhodopsins
Physical chemistry
Calorimetry
Membrane Transport Proteins
Phase Transition
Neutrons
Fourier Transform Infrared Spectroscopy
Neutron scattering
Thermodynamics
Phase transitions
Temperature
Liquids

Keywords

  • Bacteriorhodopsin
  • Differential scanning calorimetry
  • Fourier transform infrared spectroscopy
  • Hofmeister effect
  • Neutron scattering
  • Protein structural fluctuation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hofmeister ions control protein dynamics. / Szalontai, B.; Nagy, Gergely; Krumova, Sashka; Fodor, E.; Páli, T.; Taneva, Stefka G.; Garab, G.; Peters, Judith; Dér, A.

In: BBA - General Subjects, Vol. 1830, No. 10, 2013, p. 4564-4572.

Research output: Contribution to journalArticle

Szalontai, B. ; Nagy, Gergely ; Krumova, Sashka ; Fodor, E. ; Páli, T. ; Taneva, Stefka G. ; Garab, G. ; Peters, Judith ; Dér, A. / Hofmeister ions control protein dynamics. In: BBA - General Subjects. 2013 ; Vol. 1830, No. 10. pp. 4564-4572.
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