HMGB1 expression and muscle regeneration in idiopathic inflammatory myopathies and degenerative joint diseases

Karolina Cseri, János Vincze, Julianna Cseri, János Fodor, Zoltán Csernátony, L. Csernoch, K. Dankó

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The High-Mobility Group Box 1 protein (HMGB1) is a known nuclear protein which may be released from the nucleus into the cytoplasm and the extracellular space. It is believed that the mobilized HMGB1 plays role in the autoimmune processes as an alarmin, stimulating the immune response. In addition, muscle regeneration and differentiation may also be altered in the inflammatory surroundings. Biopsy specimens derived from patients with idiopathic inflammatory myopathies (IIM) such as polymyositis or dermatomyositis were compared to muscle samples from patients undergoing surgical interventions for coxarthrosis. The biopsy and surgery specimens were used for Western blot analysis, for immunohistochemical detection of HMGB1 in histological preparations and for cell culturing to examine cell proliferation and differentiation. Our data show lower HMGB1 expression, impaired proliferation and slightly altered fusion capacity in the primary cell cultures started from IIM specimens than in cultures of coxarthrotic muscles. The ratio of regenerating muscle fibres with centralised nuclei (myotubes) is lower in the IIM samples than in the coxarthrotic ones but corticosteroid treatment shifts the ratio towards the coxarthrotic value. Our data suggest that the impaired regeneration capacity should also be considered to be behind the muscle weakness in IIM patients. The role of HMGB1 as a pathogenic signal requires further investigation.

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalJournal of Muscle Research and Cell Motility
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 12 2015

Fingerprint

HMGB1 Protein
Myositis
Osteoarthritis
Muscle
Regeneration
Muscles
Biopsy
Cell culture
Hip Osteoarthritis
Dermatomyositis
Primary Cell Culture
Skeletal Muscle Fibers
Muscle Weakness
Cell proliferation
Extracellular Space
Nuclear Proteins
Surgery
Cell Differentiation
Adrenal Cortex Hormones
Cytoplasm

Keywords

  • Differentiation
  • HMGB1
  • Idiopathic inflammatory myopathy
  • Muscle regeneration
  • Myogenesis
  • Proliferation
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biochemistry

Cite this

HMGB1 expression and muscle regeneration in idiopathic inflammatory myopathies and degenerative joint diseases. / Cseri, Karolina; Vincze, János; Cseri, Julianna; Fodor, János; Csernátony, Zoltán; Csernoch, L.; Dankó, K.

In: Journal of Muscle Research and Cell Motility, Vol. 36, No. 3, 12.03.2015, p. 255-262.

Research output: Contribution to journalArticle

Cseri, Karolina ; Vincze, János ; Cseri, Julianna ; Fodor, János ; Csernátony, Zoltán ; Csernoch, L. ; Dankó, K. / HMGB1 expression and muscle regeneration in idiopathic inflammatory myopathies and degenerative joint diseases. In: Journal of Muscle Research and Cell Motility. 2015 ; Vol. 36, No. 3. pp. 255-262.
@article{35d1a72a50a44c2daf189536a31e21da,
title = "HMGB1 expression and muscle regeneration in idiopathic inflammatory myopathies and degenerative joint diseases",
abstract = "The High-Mobility Group Box 1 protein (HMGB1) is a known nuclear protein which may be released from the nucleus into the cytoplasm and the extracellular space. It is believed that the mobilized HMGB1 plays role in the autoimmune processes as an alarmin, stimulating the immune response. In addition, muscle regeneration and differentiation may also be altered in the inflammatory surroundings. Biopsy specimens derived from patients with idiopathic inflammatory myopathies (IIM) such as polymyositis or dermatomyositis were compared to muscle samples from patients undergoing surgical interventions for coxarthrosis. The biopsy and surgery specimens were used for Western blot analysis, for immunohistochemical detection of HMGB1 in histological preparations and for cell culturing to examine cell proliferation and differentiation. Our data show lower HMGB1 expression, impaired proliferation and slightly altered fusion capacity in the primary cell cultures started from IIM specimens than in cultures of coxarthrotic muscles. The ratio of regenerating muscle fibres with centralised nuclei (myotubes) is lower in the IIM samples than in the coxarthrotic ones but corticosteroid treatment shifts the ratio towards the coxarthrotic value. Our data suggest that the impaired regeneration capacity should also be considered to be behind the muscle weakness in IIM patients. The role of HMGB1 as a pathogenic signal requires further investigation.",
keywords = "Differentiation, HMGB1, Idiopathic inflammatory myopathy, Muscle regeneration, Myogenesis, Proliferation, Skeletal muscle",
author = "Karolina Cseri and J{\'a}nos Vincze and Julianna Cseri and J{\'a}nos Fodor and Zolt{\'a}n Csern{\'a}tony and L. Csernoch and K. Dank{\'o}",
year = "2015",
month = "3",
day = "12",
doi = "10.1007/s10974-015-9411-7",
language = "English",
volume = "36",
pages = "255--262",
journal = "Journal of Muscle Research and Cell Motility",
issn = "0142-4319",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - HMGB1 expression and muscle regeneration in idiopathic inflammatory myopathies and degenerative joint diseases

AU - Cseri, Karolina

AU - Vincze, János

AU - Cseri, Julianna

AU - Fodor, János

AU - Csernátony, Zoltán

AU - Csernoch, L.

AU - Dankó, K.

PY - 2015/3/12

Y1 - 2015/3/12

N2 - The High-Mobility Group Box 1 protein (HMGB1) is a known nuclear protein which may be released from the nucleus into the cytoplasm and the extracellular space. It is believed that the mobilized HMGB1 plays role in the autoimmune processes as an alarmin, stimulating the immune response. In addition, muscle regeneration and differentiation may also be altered in the inflammatory surroundings. Biopsy specimens derived from patients with idiopathic inflammatory myopathies (IIM) such as polymyositis or dermatomyositis were compared to muscle samples from patients undergoing surgical interventions for coxarthrosis. The biopsy and surgery specimens were used for Western blot analysis, for immunohistochemical detection of HMGB1 in histological preparations and for cell culturing to examine cell proliferation and differentiation. Our data show lower HMGB1 expression, impaired proliferation and slightly altered fusion capacity in the primary cell cultures started from IIM specimens than in cultures of coxarthrotic muscles. The ratio of regenerating muscle fibres with centralised nuclei (myotubes) is lower in the IIM samples than in the coxarthrotic ones but corticosteroid treatment shifts the ratio towards the coxarthrotic value. Our data suggest that the impaired regeneration capacity should also be considered to be behind the muscle weakness in IIM patients. The role of HMGB1 as a pathogenic signal requires further investigation.

AB - The High-Mobility Group Box 1 protein (HMGB1) is a known nuclear protein which may be released from the nucleus into the cytoplasm and the extracellular space. It is believed that the mobilized HMGB1 plays role in the autoimmune processes as an alarmin, stimulating the immune response. In addition, muscle regeneration and differentiation may also be altered in the inflammatory surroundings. Biopsy specimens derived from patients with idiopathic inflammatory myopathies (IIM) such as polymyositis or dermatomyositis were compared to muscle samples from patients undergoing surgical interventions for coxarthrosis. The biopsy and surgery specimens were used for Western blot analysis, for immunohistochemical detection of HMGB1 in histological preparations and for cell culturing to examine cell proliferation and differentiation. Our data show lower HMGB1 expression, impaired proliferation and slightly altered fusion capacity in the primary cell cultures started from IIM specimens than in cultures of coxarthrotic muscles. The ratio of regenerating muscle fibres with centralised nuclei (myotubes) is lower in the IIM samples than in the coxarthrotic ones but corticosteroid treatment shifts the ratio towards the coxarthrotic value. Our data suggest that the impaired regeneration capacity should also be considered to be behind the muscle weakness in IIM patients. The role of HMGB1 as a pathogenic signal requires further investigation.

KW - Differentiation

KW - HMGB1

KW - Idiopathic inflammatory myopathy

KW - Muscle regeneration

KW - Myogenesis

KW - Proliferation

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=84931567194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84931567194&partnerID=8YFLogxK

U2 - 10.1007/s10974-015-9411-7

DO - 10.1007/s10974-015-9411-7

M3 - Article

VL - 36

SP - 255

EP - 262

JO - Journal of Muscle Research and Cell Motility

JF - Journal of Muscle Research and Cell Motility

SN - 0142-4319

IS - 3

ER -