HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells

A. Jenei, Sándor Varga, L. Bene, L. Mátyus, Andrea Bodnár, Z. Bacsó, Carlo Pieri, R. Gáspár, T. Farkas, S. Damjanovich

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Abstract

Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a fraction of the MHC class II molecules was also heteroclustered with MHC class I antigens at the same hierarchical level as described by the scanning force microscopy data, after specifically and sequentially labeling the antigens with 30- and 15-nm immunogold beads. On JY cells the estimated fraction of co-clustered HLA II was 0.61, whereas that of the HLA I was 0.24. Clusterization of the antigens was detected by the deviation of their spatial distribution from the Poissonian distribution representing the random case. Fluorescence resonance energy transfer measurements also confirmed partial co-clustering of the HLA class I and II molecules at another hierarchical level characterized by the 2- to 10-nm Forster distance range and providing fine details of the molecular organization of receptors. The larger-scale topological organization of the MHC class I and II antigens may reflect underlying membrane lipid domains and may fulfill significant functions in cell-to-cell contacts and signal transduction.

Original languageEnglish
Pages (from-to)7269-7274
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number14
DOIs
Publication statusPublished - Jul 8 1997

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Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
HLA Antigens
Major Histocompatibility Complex
Cell Membrane
Atomic Force Microscopy
Antigens
Fluorescence Resonance Energy Transfer
B-Cell Lymphoma
Membrane Lipids
Cluster Analysis
Signal Transduction
Electron Microscopy

Keywords

  • Atomic force microscopy
  • Electron microscopy
  • Fluorescence resonance energy transfer
  • Major histocompatibility complex antigen
  • Receptor patterns

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells",
abstract = "Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a fraction of the MHC class II molecules was also heteroclustered with MHC class I antigens at the same hierarchical level as described by the scanning force microscopy data, after specifically and sequentially labeling the antigens with 30- and 15-nm immunogold beads. On JY cells the estimated fraction of co-clustered HLA II was 0.61, whereas that of the HLA I was 0.24. Clusterization of the antigens was detected by the deviation of their spatial distribution from the Poissonian distribution representing the random case. Fluorescence resonance energy transfer measurements also confirmed partial co-clustering of the HLA class I and II molecules at another hierarchical level characterized by the 2- to 10-nm Forster distance range and providing fine details of the molecular organization of receptors. The larger-scale topological organization of the MHC class I and II antigens may reflect underlying membrane lipid domains and may fulfill significant functions in cell-to-cell contacts and signal transduction.",
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author = "A. Jenei and S{\'a}ndor Varga and L. Bene and L. M{\'a}tyus and Andrea Bodn{\'a}r and Z. Bacs{\'o} and Carlo Pieri and R. G{\'a}sp{\'a}r and T. Farkas and S. Damjanovich",
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T1 - HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells

AU - Jenei, A.

AU - Varga, Sándor

AU - Bene, L.

AU - Mátyus, L.

AU - Bodnár, Andrea

AU - Bacsó, Z.

AU - Pieri, Carlo

AU - Gáspár, R.

AU - Farkas, T.

AU - Damjanovich, S.

PY - 1997/7/8

Y1 - 1997/7/8

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AB - Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a fraction of the MHC class II molecules was also heteroclustered with MHC class I antigens at the same hierarchical level as described by the scanning force microscopy data, after specifically and sequentially labeling the antigens with 30- and 15-nm immunogold beads. On JY cells the estimated fraction of co-clustered HLA II was 0.61, whereas that of the HLA I was 0.24. Clusterization of the antigens was detected by the deviation of their spatial distribution from the Poissonian distribution representing the random case. Fluorescence resonance energy transfer measurements also confirmed partial co-clustering of the HLA class I and II molecules at another hierarchical level characterized by the 2- to 10-nm Forster distance range and providing fine details of the molecular organization of receptors. The larger-scale topological organization of the MHC class I and II antigens may reflect underlying membrane lipid domains and may fulfill significant functions in cell-to-cell contacts and signal transduction.

KW - Atomic force microscopy

KW - Electron microscopy

KW - Fluorescence resonance energy transfer

KW - Major histocompatibility complex antigen

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