HIV-1 induces human monocyte-derived macrophages to produce C3 and to fix C3 on their surface

Zsuzsa Bajtay, Laco Kacani, Anna Erdei, Manfred P. Dierich

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Complement components, particularly C3, are known to be involved in the pathogenesis of AIDS and macrophages may serve as a source of C3 at sites of infection. We investigated whether the interaction between HIV-1 and monocytes has any effect on C3 production by the cells. Monocytes isolated from the blood of healthy volunteers were incubated with monocytotropic and T lymphocytotropic HIV-1 strains or with recombinant gp 160 and cultured in serum-free medium up to 7 days. Supernatants were tested for secreted C3 by enzyme-linked immunosorbent assay. Our data show that monocytes cultured with either the monocytotropic or the T lymphocytotropic HIV-1 strains produce C3 in large amounts. The effect of both viruses is dose dependent and the amount of C3 induced by HIV was up to 20-fold higher than in the control samples. C3 production was also enhanced by gp160, the envelope protein of the virus. Secretion of IL-6 by the cells was also measured and found to be elevated up to threefold as a consequence of the interaction with the virus. HIV-1- activated monocyte-derived macrophages acquired the capacity to cleave exogenous C3 and to fix generated C3 fragments on their cell membrane.

Original languageEnglish
Pages (from-to)463-468
Number of pages6
JournalJournal of Leukocyte Biology
Volume63
Issue number4
DOIs
Publication statusPublished - Apr 1998

Keywords

  • C3 deposition
  • Complement activation
  • Interleukin-6

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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