A tüdó tuberkulotikus granulomájának szövettani és immunhisztokémiai szerkezete antituberkulotikummal kezelt és kezeletlen esetekben.

Translated title of the contribution: Histological and immunohistochemical structure of pulmonary tuberculotic granulomas in untreated cases and cases treated with antitubercular drugs

József Furák, Imre Troján, Tamás Szóke, L. Tiszlavicz, K. Boda, Adám Balogh, E. Rőth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

INTRODUCTION: The changes occurring in response to antituberculotic treatment and immune defence were studied in human tuberculotic granulomas. AIMS: To compare the possibilities of detection of Mycobacterium tuberculosis with the Ziehl-Neelsen staining technique and with an immunohistochemical method, and to assess the roles of lymphocytes and heat-shock protein 70. METHOD: 40 patients who had undergone lung resection (the postoperative histology confirmed tuberculosis) were divided into two equal groups, on the basis of whether they had received antituberculotic treatment preoperatively (group I) or not (group II). Customary histology was used to determine the Langhans cells, epitheloid cells and lymphocytes, and an immunohistochemical method was then applied to examine the heat-shock protein 70 production of these cells and the normal lung. The lymphocytes were divided into CD4+ T-helper, CD8+ T-cytotoxic and CD20+ B cells by means of immune examinations. M. tuberculosis was demonstrated by an immunohistochemical method, with antibody against the wall protein. RESULTS: Heat-shock protein 70 was produced by 17.6% of the Langhans cells and 94.4% of the epitheloid cells in group I, and by 100% of both cell types in group II. The bacterium could be detected in 40% of the total number of cases with acid-fast staining, and in 85% by immunohistochemistry. There was no significant difference in the qualitative distribution of the lymphocytes in the granulomas in groups I and II. The heat-shock protein 70 levels of the tuberculotic granuloma and the normal lung were significantly higher in group II. CONCLUSIONS: The production of heat-shock protein 70 is more enhanced in untreated tuberculotic cases. On the basis of their heat-shock protein 70 production, the authors assume that a majority of the Langhans cells have a resting protective function in medically treated cases. Independently of the stage of the infection and of the use or not of antituberculotic treatment, the number of lymphocytes participating in the immune defence is constant. By means of immunohistochemical examination of the wall protein of M. tuberculosis, the presence of the tuberculotic disease can be demonstrated with high reliability.

Original languageHungarian
Pages (from-to)1347-1352
Number of pages6
JournalOrvosi Hetilap
Volume144
Issue number27
Publication statusPublished - Jul 6 2003

Fingerprint

Antitubercular Agents
HSP70 Heat-Shock Proteins
Granuloma
Lung
Mycobacterium tuberculosis
Lymphocytes
Histology
Staining and Labeling
Lymphocyte Count
Tuberculosis
Proteins
B-Lymphocytes
Therapeutics
Immunohistochemistry
Bacteria
Acids
Antibodies
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A tüdó tuberkulotikus granulomájának szövettani és immunhisztokémiai szerkezete antituberkulotikummal kezelt és kezeletlen esetekben. / Furák, József; Troján, Imre; Szóke, Tamás; Tiszlavicz, L.; Boda, K.; Balogh, Adám; Rőth, E.

In: Orvosi Hetilap, Vol. 144, No. 27, 06.07.2003, p. 1347-1352.

Research output: Contribution to journalArticle

@article{8b9c9564635c41dc933ae0edfa0f1221,
title = "A t{\"u}d{\'o} tuberkulotikus granulom{\'a}j{\'a}nak sz{\"o}vettani {\'e}s immunhisztok{\'e}miai szerkezete antituberkulotikummal kezelt {\'e}s kezeletlen esetekben.",
abstract = "INTRODUCTION: The changes occurring in response to antituberculotic treatment and immune defence were studied in human tuberculotic granulomas. AIMS: To compare the possibilities of detection of Mycobacterium tuberculosis with the Ziehl-Neelsen staining technique and with an immunohistochemical method, and to assess the roles of lymphocytes and heat-shock protein 70. METHOD: 40 patients who had undergone lung resection (the postoperative histology confirmed tuberculosis) were divided into two equal groups, on the basis of whether they had received antituberculotic treatment preoperatively (group I) or not (group II). Customary histology was used to determine the Langhans cells, epitheloid cells and lymphocytes, and an immunohistochemical method was then applied to examine the heat-shock protein 70 production of these cells and the normal lung. The lymphocytes were divided into CD4+ T-helper, CD8+ T-cytotoxic and CD20+ B cells by means of immune examinations. M. tuberculosis was demonstrated by an immunohistochemical method, with antibody against the wall protein. RESULTS: Heat-shock protein 70 was produced by 17.6{\%} of the Langhans cells and 94.4{\%} of the epitheloid cells in group I, and by 100{\%} of both cell types in group II. The bacterium could be detected in 40{\%} of the total number of cases with acid-fast staining, and in 85{\%} by immunohistochemistry. There was no significant difference in the qualitative distribution of the lymphocytes in the granulomas in groups I and II. The heat-shock protein 70 levels of the tuberculotic granuloma and the normal lung were significantly higher in group II. CONCLUSIONS: The production of heat-shock protein 70 is more enhanced in untreated tuberculotic cases. On the basis of their heat-shock protein 70 production, the authors assume that a majority of the Langhans cells have a resting protective function in medically treated cases. Independently of the stage of the infection and of the use or not of antituberculotic treatment, the number of lymphocytes participating in the immune defence is constant. By means of immunohistochemical examination of the wall protein of M. tuberculosis, the presence of the tuberculotic disease can be demonstrated with high reliability.",
author = "J{\'o}zsef Fur{\'a}k and Imre Troj{\'a}n and Tam{\'a}s Sz{\'o}ke and L. Tiszlavicz and K. Boda and Ad{\'a}m Balogh and E. Rőth",
year = "2003",
month = "7",
day = "6",
language = "Hungarian",
volume = "144",
pages = "1347--1352",
journal = "Orvosi Hetilap",
issn = "0030-6002",
publisher = "Akademiai Kiado",
number = "27",

}

TY - JOUR

T1 - A tüdó tuberkulotikus granulomájának szövettani és immunhisztokémiai szerkezete antituberkulotikummal kezelt és kezeletlen esetekben.

AU - Furák, József

AU - Troján, Imre

AU - Szóke, Tamás

AU - Tiszlavicz, L.

AU - Boda, K.

AU - Balogh, Adám

AU - Rőth, E.

PY - 2003/7/6

Y1 - 2003/7/6

N2 - INTRODUCTION: The changes occurring in response to antituberculotic treatment and immune defence were studied in human tuberculotic granulomas. AIMS: To compare the possibilities of detection of Mycobacterium tuberculosis with the Ziehl-Neelsen staining technique and with an immunohistochemical method, and to assess the roles of lymphocytes and heat-shock protein 70. METHOD: 40 patients who had undergone lung resection (the postoperative histology confirmed tuberculosis) were divided into two equal groups, on the basis of whether they had received antituberculotic treatment preoperatively (group I) or not (group II). Customary histology was used to determine the Langhans cells, epitheloid cells and lymphocytes, and an immunohistochemical method was then applied to examine the heat-shock protein 70 production of these cells and the normal lung. The lymphocytes were divided into CD4+ T-helper, CD8+ T-cytotoxic and CD20+ B cells by means of immune examinations. M. tuberculosis was demonstrated by an immunohistochemical method, with antibody against the wall protein. RESULTS: Heat-shock protein 70 was produced by 17.6% of the Langhans cells and 94.4% of the epitheloid cells in group I, and by 100% of both cell types in group II. The bacterium could be detected in 40% of the total number of cases with acid-fast staining, and in 85% by immunohistochemistry. There was no significant difference in the qualitative distribution of the lymphocytes in the granulomas in groups I and II. The heat-shock protein 70 levels of the tuberculotic granuloma and the normal lung were significantly higher in group II. CONCLUSIONS: The production of heat-shock protein 70 is more enhanced in untreated tuberculotic cases. On the basis of their heat-shock protein 70 production, the authors assume that a majority of the Langhans cells have a resting protective function in medically treated cases. Independently of the stage of the infection and of the use or not of antituberculotic treatment, the number of lymphocytes participating in the immune defence is constant. By means of immunohistochemical examination of the wall protein of M. tuberculosis, the presence of the tuberculotic disease can be demonstrated with high reliability.

AB - INTRODUCTION: The changes occurring in response to antituberculotic treatment and immune defence were studied in human tuberculotic granulomas. AIMS: To compare the possibilities of detection of Mycobacterium tuberculosis with the Ziehl-Neelsen staining technique and with an immunohistochemical method, and to assess the roles of lymphocytes and heat-shock protein 70. METHOD: 40 patients who had undergone lung resection (the postoperative histology confirmed tuberculosis) were divided into two equal groups, on the basis of whether they had received antituberculotic treatment preoperatively (group I) or not (group II). Customary histology was used to determine the Langhans cells, epitheloid cells and lymphocytes, and an immunohistochemical method was then applied to examine the heat-shock protein 70 production of these cells and the normal lung. The lymphocytes were divided into CD4+ T-helper, CD8+ T-cytotoxic and CD20+ B cells by means of immune examinations. M. tuberculosis was demonstrated by an immunohistochemical method, with antibody against the wall protein. RESULTS: Heat-shock protein 70 was produced by 17.6% of the Langhans cells and 94.4% of the epitheloid cells in group I, and by 100% of both cell types in group II. The bacterium could be detected in 40% of the total number of cases with acid-fast staining, and in 85% by immunohistochemistry. There was no significant difference in the qualitative distribution of the lymphocytes in the granulomas in groups I and II. The heat-shock protein 70 levels of the tuberculotic granuloma and the normal lung were significantly higher in group II. CONCLUSIONS: The production of heat-shock protein 70 is more enhanced in untreated tuberculotic cases. On the basis of their heat-shock protein 70 production, the authors assume that a majority of the Langhans cells have a resting protective function in medically treated cases. Independently of the stage of the infection and of the use or not of antituberculotic treatment, the number of lymphocytes participating in the immune defence is constant. By means of immunohistochemical examination of the wall protein of M. tuberculosis, the presence of the tuberculotic disease can be demonstrated with high reliability.

UR - http://www.scopus.com/inward/record.url?scp=0141499857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141499857&partnerID=8YFLogxK

M3 - Article

C2 - 12908148

AN - SCOPUS:0141499857

VL - 144

SP - 1347

EP - 1352

JO - Orvosi Hetilap

JF - Orvosi Hetilap

SN - 0030-6002

IS - 27

ER -