Histologic findings after gastrocystoplasty in rabbits

Peter Vajda, Andrew B. Pinter, Tamas Magyarlaki, Attila M. Vastyan, Zsolt Juhasz, Zsolt Oberritter, Khaled Fathi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: The aim of this study was to investigate the long-term histologic changes after bladder augmentation with gastric segment in an animal subject. Materials and Methods: Gastrocystoplasty was performed in 13 young, 3-month-old male rabbits. Open biopsies were taken from the native bladder and the gastric segment preoperatively and at 3, 6, and 12 months postoperatively. Sections were examined with H&E and periodic acid-Schiff (PAS) staining. Indirect immune peroxidase method was additionally applied to detect the carcinoembrionic antigen, the proliferative activity, and the gene for the tumor protein p53 in the epithelium. Results: On the native bladder, at the 3-month follow-up, polyps, mucosal edema, submucosal fibrosis, and squamous cell metaplasia were detected, which did not change during the follow-up. On the gastric segment, at the 3-month follow-up, parietal cell hyperplasia and inflammatory mucosal overgrowth were detected; at the 6-month follow-up, inflammation or atrophy of the gastric mucosa and colonic-type metaplasia was found. These alterations remained unchanged during later course of follow-up. Neither dysplasia nor malignancy was observed during the 12-month follow-up. Conclusions: The present study supports the clinical observations of low cancer risk after gastrocystoplasty and may indicate different effect of gastric secretion on uroepithelium and that of urine on gastric mucosa.

Original languageEnglish
Pages (from-to)1470-1474
Number of pages5
JournalJournal of Pediatric Surgery
Volume40
Issue number9
DOIs
Publication statusPublished - Sep 1 2005

Keywords

  • Gastrocystoplasty
  • Long-term follow-up
  • Malignancy
  • Rabbits

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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  • Cite this

    Vajda, P., Pinter, A. B., Magyarlaki, T., Vastyan, A. M., Juhasz, Z., Oberritter, Z., & Fathi, K. (2005). Histologic findings after gastrocystoplasty in rabbits. Journal of Pediatric Surgery, 40(9), 1470-1474. https://doi.org/10.1016/j.jpedsurg.2005.05.047