Histochemical study of non specific acid and alkaline phosphatase, adenosine triphosphatase and lactate dehydrogenase in the dog liver perfused in vitro

J. Faller, P. Kupcsulik, S. Karacsonyi

Research output: Contribution to journalArticle

Abstract

In dog liver, perfused in vitro at 4° and 38° C, the following were studied histochemically: the influence of ischemia on non specific alkaline and acid phosphatase, and adenosine triphosphatase and lactate dehydrogenase activity. A 30 min ischemia caused essential changes in enzyme activity. Hypothermic perfusion was the most suitable procedure for liver preservation, although the results obtained under hypothermia without perfusion were almost as good. Histochemical signs of early mitochondrial lesions were frequent.

Original languageEnglish
Pages (from-to)313-326
Number of pages14
JournalActa Chirurgica Academiae Scientiarum Hungaricae
Volume15
Issue number3
Publication statusPublished - 1974

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Acid Phosphatase
L-Lactate Dehydrogenase
Alkaline Phosphatase
Adenosine Triphosphatases
Ischemia
Perfusion
Dogs
Liver
Hypothermia
Enzymes
In Vitro Techniques

ASJC Scopus subject areas

  • Surgery

Cite this

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abstract = "In dog liver, perfused in vitro at 4° and 38° C, the following were studied histochemically: the influence of ischemia on non specific alkaline and acid phosphatase, and adenosine triphosphatase and lactate dehydrogenase activity. A 30 min ischemia caused essential changes in enzyme activity. Hypothermic perfusion was the most suitable procedure for liver preservation, although the results obtained under hypothermia without perfusion were almost as good. Histochemical signs of early mitochondrial lesions were frequent.",
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