Histochemical demonstration of copper in normal rat brain and spinal cord - Evidence of localization in glial cells

P. Szerdahelyi, P. Kása

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The high sensitivity of the magnesium-dithizonate silver-dithizonate (MDSD) staining procedure makes this method very suitable for the histochemical localization of copper in different regions of the central nervous system of adult rats. In the telencephalon (bulbus olfactorius, nucleus caudatus-putamen, septum pellucidum and are dentata), diencephalon (nucleus habenulae medialis, nuclei of the hypothalamus in the vicinity of the third ventricle, and corpus mamillare), mesencephalon (substantia nigra), cerebellum (mainly in the nodulus), pons (locus coeruleus, nucleus vestibularis), medulla oblongata (nucleus tractus solitarii) and spinal cord, the glial cells exhibit specific copper staining. The glial cells of some circumventricular organs (e.g. the subfornical organ) are also stained using the MDSD method. The significant staining observed in whitematter glial cells (e.g. in the corpus callosum, cerebellum and spinal cord) further indicates the very high sensitivity of this method. In glial cells of the same regions, the presence of copper can likewise be demonstrated using the modified sulphide silver method. On the basis of the present histochemical results, it is suggested that copper may play an important role in the normal physiological functioning of glial cells and also, via glia-neuron interactions, in neuronal processes.

Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalHistochemistry
Volume85
Issue number4
DOIs
Publication statusPublished - Jul 1986

Fingerprint

Neuroglia
Copper
Spinal Cord
Brain
Cerebellum
Magnesium
Septum Pellucidum
Subfornical Organ
Staining and Labeling
Habenula
Diencephalon
Telencephalon
Medulla Oblongata
Silver Staining
Third Ventricle
Solitary Nucleus
Locus Coeruleus
Pons
Corpus Callosum
Caudate Nucleus

ASJC Scopus subject areas

  • Anatomy
  • Medicine(all)

Cite this

Histochemical demonstration of copper in normal rat brain and spinal cord - Evidence of localization in glial cells. / Szerdahelyi, P.; Kása, P.

In: Histochemistry, Vol. 85, No. 4, 07.1986, p. 341-347.

Research output: Contribution to journalArticle

@article{cfe6deea784140a7ab484d8c33132c97,
title = "Histochemical demonstration of copper in normal rat brain and spinal cord - Evidence of localization in glial cells",
abstract = "The high sensitivity of the magnesium-dithizonate silver-dithizonate (MDSD) staining procedure makes this method very suitable for the histochemical localization of copper in different regions of the central nervous system of adult rats. In the telencephalon (bulbus olfactorius, nucleus caudatus-putamen, septum pellucidum and are dentata), diencephalon (nucleus habenulae medialis, nuclei of the hypothalamus in the vicinity of the third ventricle, and corpus mamillare), mesencephalon (substantia nigra), cerebellum (mainly in the nodulus), pons (locus coeruleus, nucleus vestibularis), medulla oblongata (nucleus tractus solitarii) and spinal cord, the glial cells exhibit specific copper staining. The glial cells of some circumventricular organs (e.g. the subfornical organ) are also stained using the MDSD method. The significant staining observed in whitematter glial cells (e.g. in the corpus callosum, cerebellum and spinal cord) further indicates the very high sensitivity of this method. In glial cells of the same regions, the presence of copper can likewise be demonstrated using the modified sulphide silver method. On the basis of the present histochemical results, it is suggested that copper may play an important role in the normal physiological functioning of glial cells and also, via glia-neuron interactions, in neuronal processes.",
author = "P. Szerdahelyi and P. K{\'a}sa",
year = "1986",
month = "7",
doi = "10.1007/BF00493487",
language = "English",
volume = "85",
pages = "341--347",
journal = "Histochemie. Histochemistry. Histochimie",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Histochemical demonstration of copper in normal rat brain and spinal cord - Evidence of localization in glial cells

AU - Szerdahelyi, P.

AU - Kása, P.

PY - 1986/7

Y1 - 1986/7

N2 - The high sensitivity of the magnesium-dithizonate silver-dithizonate (MDSD) staining procedure makes this method very suitable for the histochemical localization of copper in different regions of the central nervous system of adult rats. In the telencephalon (bulbus olfactorius, nucleus caudatus-putamen, septum pellucidum and are dentata), diencephalon (nucleus habenulae medialis, nuclei of the hypothalamus in the vicinity of the third ventricle, and corpus mamillare), mesencephalon (substantia nigra), cerebellum (mainly in the nodulus), pons (locus coeruleus, nucleus vestibularis), medulla oblongata (nucleus tractus solitarii) and spinal cord, the glial cells exhibit specific copper staining. The glial cells of some circumventricular organs (e.g. the subfornical organ) are also stained using the MDSD method. The significant staining observed in whitematter glial cells (e.g. in the corpus callosum, cerebellum and spinal cord) further indicates the very high sensitivity of this method. In glial cells of the same regions, the presence of copper can likewise be demonstrated using the modified sulphide silver method. On the basis of the present histochemical results, it is suggested that copper may play an important role in the normal physiological functioning of glial cells and also, via glia-neuron interactions, in neuronal processes.

AB - The high sensitivity of the magnesium-dithizonate silver-dithizonate (MDSD) staining procedure makes this method very suitable for the histochemical localization of copper in different regions of the central nervous system of adult rats. In the telencephalon (bulbus olfactorius, nucleus caudatus-putamen, septum pellucidum and are dentata), diencephalon (nucleus habenulae medialis, nuclei of the hypothalamus in the vicinity of the third ventricle, and corpus mamillare), mesencephalon (substantia nigra), cerebellum (mainly in the nodulus), pons (locus coeruleus, nucleus vestibularis), medulla oblongata (nucleus tractus solitarii) and spinal cord, the glial cells exhibit specific copper staining. The glial cells of some circumventricular organs (e.g. the subfornical organ) are also stained using the MDSD method. The significant staining observed in whitematter glial cells (e.g. in the corpus callosum, cerebellum and spinal cord) further indicates the very high sensitivity of this method. In glial cells of the same regions, the presence of copper can likewise be demonstrated using the modified sulphide silver method. On the basis of the present histochemical results, it is suggested that copper may play an important role in the normal physiological functioning of glial cells and also, via glia-neuron interactions, in neuronal processes.

UR - http://www.scopus.com/inward/record.url?scp=0022459582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022459582&partnerID=8YFLogxK

U2 - 10.1007/BF00493487

DO - 10.1007/BF00493487

M3 - Article

C2 - 2428777

AN - SCOPUS:0022459582

VL - 85

SP - 341

EP - 347

JO - Histochemie. Histochemistry. Histochimie

JF - Histochemie. Histochemistry. Histochimie

SN - 0948-6143

IS - 4

ER -