Histidine decarboxylase in peripheral lymphocytes of healthy individuals and chronic lymphoid leukemia patients possible involvement of intracellular histamine in the regulation of lymphocyte proliferation

Márta Bencsáth, K. Pálóczi, C. Szalai, Andrea Szenthe, Júlia Szeberényi, A. Falus

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Abstract

Histidine decarboxylase (HDC), the only enzyme capable of synthesis of histamine, has been found in many proliferating cells and tissues suggesting a role of histamine in cellular proliferation. In this study expression of HDC and the significance of histamine in the proliferation of peripheral lymphocytes of five healthy persons and six patients with chronic lymphoid leukemia (CLL) was examined. Expression of HDC mRNA and HDC protein was proved by reverse transcriptase polymerase chain reaction and by immunoblot, respectively. The role of histamine was studied in proliferation assays in the presence of irreversible inhibitor of the HDC (alpha- fluoromethylhistidine-FMH) and also by competing for the intracellular binding sites of histamine using N,N-diethyl-2,4phenylmethyl-phenoxy- ethanamine-HCl (DPPE). By inhibiting the HDC enzyme activity by FMH and blocking the intracellular action of histamine by DPPE, a significant decrease in cell proliferation was observed in mitogen stimulated lymphocytes of healthy donors. In CLL patients the proliferation of leukemic lymphocytes was significantly inhibited by blocking the binding of histamine to intracellular binding sites by DPPE, but not by FMH inhibiting only the de novo histamine formation. The observations suggest that HDC has functional relevance in lymphocytes, since mitogen induced lymphocyte proliferation of healthy donors is mainly enhanced by de novo synthesis and subsequent action of intracellular histamine. Alternatively, in constitutively proliferating chronic lymphoid leukemia cells we suggest that the preformed pool but not the de novo synthesized intracellular histamine interferes with cellular proliferation.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalPathology and Oncology Research
Volume4
Issue number2
Publication statusPublished - 1998

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Histidine Decarboxylase
Lymphoid Leukemia
Histamine
Lymphocytes
Cell Proliferation
Mitogens
Tissue Donors
Histamine Receptors
Enzymes
Reverse Transcriptase Polymerase Chain Reaction
Binding Sites

Keywords

  • Cell proliferation
  • Histamine
  • Leukemia
  • Lymphocyte

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

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title = "Histidine decarboxylase in peripheral lymphocytes of healthy individuals and chronic lymphoid leukemia patients possible involvement of intracellular histamine in the regulation of lymphocyte proliferation",
abstract = "Histidine decarboxylase (HDC), the only enzyme capable of synthesis of histamine, has been found in many proliferating cells and tissues suggesting a role of histamine in cellular proliferation. In this study expression of HDC and the significance of histamine in the proliferation of peripheral lymphocytes of five healthy persons and six patients with chronic lymphoid leukemia (CLL) was examined. Expression of HDC mRNA and HDC protein was proved by reverse transcriptase polymerase chain reaction and by immunoblot, respectively. The role of histamine was studied in proliferation assays in the presence of irreversible inhibitor of the HDC (alpha- fluoromethylhistidine-FMH) and also by competing for the intracellular binding sites of histamine using N,N-diethyl-2,4phenylmethyl-phenoxy- ethanamine-HCl (DPPE). By inhibiting the HDC enzyme activity by FMH and blocking the intracellular action of histamine by DPPE, a significant decrease in cell proliferation was observed in mitogen stimulated lymphocytes of healthy donors. In CLL patients the proliferation of leukemic lymphocytes was significantly inhibited by blocking the binding of histamine to intracellular binding sites by DPPE, but not by FMH inhibiting only the de novo histamine formation. The observations suggest that HDC has functional relevance in lymphocytes, since mitogen induced lymphocyte proliferation of healthy donors is mainly enhanced by de novo synthesis and subsequent action of intracellular histamine. Alternatively, in constitutively proliferating chronic lymphoid leukemia cells we suggest that the preformed pool but not the de novo synthesized intracellular histamine interferes with cellular proliferation.",
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author = "M{\'a}rta Bencs{\'a}th and K. P{\'a}l{\'o}czi and C. Szalai and Andrea Szenthe and J{\'u}lia Szeber{\'e}nyi and A. Falus",
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T1 - Histidine decarboxylase in peripheral lymphocytes of healthy individuals and chronic lymphoid leukemia patients possible involvement of intracellular histamine in the regulation of lymphocyte proliferation

AU - Bencsáth, Márta

AU - Pálóczi, K.

AU - Szalai, C.

AU - Szenthe, Andrea

AU - Szeberényi, Júlia

AU - Falus, A.

PY - 1998

Y1 - 1998

N2 - Histidine decarboxylase (HDC), the only enzyme capable of synthesis of histamine, has been found in many proliferating cells and tissues suggesting a role of histamine in cellular proliferation. In this study expression of HDC and the significance of histamine in the proliferation of peripheral lymphocytes of five healthy persons and six patients with chronic lymphoid leukemia (CLL) was examined. Expression of HDC mRNA and HDC protein was proved by reverse transcriptase polymerase chain reaction and by immunoblot, respectively. The role of histamine was studied in proliferation assays in the presence of irreversible inhibitor of the HDC (alpha- fluoromethylhistidine-FMH) and also by competing for the intracellular binding sites of histamine using N,N-diethyl-2,4phenylmethyl-phenoxy- ethanamine-HCl (DPPE). By inhibiting the HDC enzyme activity by FMH and blocking the intracellular action of histamine by DPPE, a significant decrease in cell proliferation was observed in mitogen stimulated lymphocytes of healthy donors. In CLL patients the proliferation of leukemic lymphocytes was significantly inhibited by blocking the binding of histamine to intracellular binding sites by DPPE, but not by FMH inhibiting only the de novo histamine formation. The observations suggest that HDC has functional relevance in lymphocytes, since mitogen induced lymphocyte proliferation of healthy donors is mainly enhanced by de novo synthesis and subsequent action of intracellular histamine. Alternatively, in constitutively proliferating chronic lymphoid leukemia cells we suggest that the preformed pool but not the de novo synthesized intracellular histamine interferes with cellular proliferation.

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