Histidine decarboxylase expression in human melanoma

Mary Haak-Frendscho, Zsuzsa Darvas, Hargita Hegyesi, Sarolta Kárpáti, Randall L. Hoffman, Valéria László, Márta Bencsáth, Csaba Szalai, József Fürész, József Timár, Zsuzsanna Bata-Csörgõ, Gábor Szabad, Andor Pivarcsi, Éva Pállinger, Lajos Kemény, Attila Horváth, Attila Dobozy, András Falus

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Histamine has been implicated as one of the mediators involved in regulation of proliferation in both normal and neoplastic tissues. Histidine decarboxylase, the only enzyme that catalyzes the formation of histamine from L-histidine, is an essential regulator of histamine levels. In this study, we investigated the gene and protein expression of histidine decarboxylase in melanoma. Reverse transcriptase polymerase chain reaction and in situ hybridization studies of WM-35, WM-983/B, HT-168, and M1 human melanoma cell lines both resulted in positive signals for histidine decarboxylase messenger RNA. A polyclonal chicken antibody was developed against human histidine decarboxylase and protein expression was confirmed by western blot analysis of the cell lysates, revealing a predominant immunoreactive band at approximately 54kDa corresponding to monomeric histidine decarboxylase. Protein expression of histidine decarboxylase was also shown by flow cytometric analysis and strong punctate cytoplasmic staining of melanoma cell lines. Moreover, both primary and metastatic human melanoma tissues were brightly stained for histidine decarboxylase. When compared with the very weak or no reactions on cultivated human melanocytes both western blot and immunohistochemical studies showed much stronger histidine decarboxylase expression in melanoma cells. These findings suggest that expression of histidine decarboxylase is elevated in human melanoma.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalJournal of Investigative Dermatology
Volume115
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Antibody
  • Histamine
  • Histidine decarboxylase
  • Melanoma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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