Histamine deficiency suppresses murine haptoglobin production and modifies hepatic protein tyrosine phosphorylation

K. Hegyi, A. K. Fülöp, S. Tóth, E. Búzás, T. Watanabe, H. Ohtsu, A. Ichikawa, A. Nagy, A. Falus

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Histidine decarboxylase (HDC) synthesizes endogenous histamine from histidine in mammals. HDC-deficient mice (HDC-/-), if kept on a histamine-free diet, have no histamine in their tissues. HDC-/-mice show multiple phenotypes. In this study we show that both the constitutively expressed and turpentine-induced level of an acute-phase protein, haptoglobin, is significantly lower in the serum of HDC-/- mice compared to that of wild-type animals. This effect was abolished if HDC gene-targeted mice received histamine-rich food. No differences were found when lipopolysaccharide (LPS) was used to induce the acute-phase reaction. Using specific antibodies to phosphorylated tyrosine, we showed that protein tyrosine phosphorylation (Y-P) of ∼ 50- and 26- to 27-kDa liver proteins is significantly decreased in HDC-/- mice, but that the difference was largely diminished if the animals were kept on a histamine-rich diet, suggesting that the phenotype with lower haptoglobin production is diet inducible. Upon in vivo treatment with LPS, Y-P band intensity decreased, regardless of the presence or absence of histamine. Identification of elements of the signalling pathway with decreased phosphorylation may elucidate the molecular background of the effect of endogenous histamine in the hepatic acute-phase reaction.

Original languageEnglish
Pages (from-to)850-854
Number of pages5
JournalCellular and Molecular Life Sciences
Volume58
Issue number5-6
Publication statusPublished - 2001

Fingerprint

Histidine Decarboxylase
Phosphorylation
Haptoglobins
Histamine
Tyrosine
Liver
Nutrition
Proteins
Acute-Phase Reaction
Diet
Lipopolysaccharides
Animals
Turpentine
Phenotype
Wild Animals
Mammals
Acute-Phase Proteins
Histidine
Genes
Tissue

Keywords

  • Acute phase protein
  • Haptoglobin
  • Histamine
  • Knockout
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Histamine deficiency suppresses murine haptoglobin production and modifies hepatic protein tyrosine phosphorylation. / Hegyi, K.; Fülöp, A. K.; Tóth, S.; Búzás, E.; Watanabe, T.; Ohtsu, H.; Ichikawa, A.; Nagy, A.; Falus, A.

In: Cellular and Molecular Life Sciences, Vol. 58, No. 5-6, 2001, p. 850-854.

Research output: Contribution to journalArticle

Hegyi, K. ; Fülöp, A. K. ; Tóth, S. ; Búzás, E. ; Watanabe, T. ; Ohtsu, H. ; Ichikawa, A. ; Nagy, A. ; Falus, A. / Histamine deficiency suppresses murine haptoglobin production and modifies hepatic protein tyrosine phosphorylation. In: Cellular and Molecular Life Sciences. 2001 ; Vol. 58, No. 5-6. pp. 850-854.
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