Hip-Arg-Phe-, Hip-Phe-Arg- and Hip-His-Leu-cleaving dipeptidyl carboxypeptidases in human adrenal tumors

A. Rónai, J. Lengyel, T. Nagyt, G. Orosz, V. Adlesz, K. Rácz, K. Magyar

Research output: Contribution to journalArticle

Abstract

Hip-Arg-Phe-, Hip-Phe-Arg- and Hip-His-Leu-cleaving dipeptidyl carboxypeptidase activities were measured in the supernatant (S2) and pellet (P2) fractions obtained by ultracentrifugation of human adrenal tumor preparations. Negligible enzyme activity was found in cortical tumor whereas highly significant activities were present in the P2 fractions of the two pheochromocytoma specimens. The hydrolysis rates, expressed in terms of the percent of: added substrate were 58-66%/60 min for Hip-Phe-Arg, 55-58%/60 min for Hip-Arg-Phe and 19-30%/60 min for Hip-His-Leu. The angiotensin- converting enzyme inhibitor, captopril, differentially inhibited the enzyme splitting Hip-His-Leu versus the one cleaving Hip-Arg-Phe; Hip-Phe-Arg is probably the substrate of both. It is concluded that the Hip-Arg-Phe-cleaving enzyme in adrenomedullary tumor is probably identical to the purportedly novel dipeptidyl carboxypeptidase that we detected earlier in rabbit ear artery wall, which converts (Met5)-enkephalin-Arg6, Phe7 to (Met5)- enkephalin.

Original languageEnglish
Pages (from-to)585-588
Number of pages4
JournalNeuropeptides
Volume31
Issue number6
DOIs
Publication statusPublished - 1997

Fingerprint

phenylalanylarginine
Glandular and Epithelial Neoplasms
Hip
Tumors
Enkephalins
Captopril
Enzyme activity
Substrates
Enzymes
Angiotensin-Converting Enzyme Inhibitors
Hydrolysis
Ultracentrifugation
Pheochromocytoma
dipeptidyl carboxypeptidase
hippuryl-histidyl-leucine
Ear
Neoplasms
Arteries
Rabbits

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Neurology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Biochemistry

Cite this

Hip-Arg-Phe-, Hip-Phe-Arg- and Hip-His-Leu-cleaving dipeptidyl carboxypeptidases in human adrenal tumors. / Rónai, A.; Lengyel, J.; Nagyt, T.; Orosz, G.; Adlesz, V.; Rácz, K.; Magyar, K.

In: Neuropeptides, Vol. 31, No. 6, 1997, p. 585-588.

Research output: Contribution to journalArticle

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AU - Nagyt, T.

AU - Orosz, G.

AU - Adlesz, V.

AU - Rácz, K.

AU - Magyar, K.

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