The physical map of human adenovirus type 1 DNA was constructed with the HindIII restriction enzyme. Direct comparison of the DNA fragments with those of adenovirus type 2 revealed that the genome of adenovirus type 1 is 200 to 300 base pairs longer. The differences are located outside the region of the inverted terminal repetition within three distinct loci. Fragment HindIII-F of type 1 DNA which is presumed to carry all the genes responsible for in vitro transformation can be separated without considerable contamination by a single electrophoretic step.
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