L929 and WEHI tumor cell lines were genetically modified to constitutively express the Saccharomyces cerevisiae Ole 1 gene, coding for the Δ9-desaturase enzyme. These cells exhibit an increased ratio of monounsaturated fatty acids in their membrane phospholipids paralleled by an overall decrease in the membrane molecular order and a highly increased tumor necrosis factor-α (TNF) sensitivity. The TNF-α signaling cascade involves events, like receptor clustering and cleavage of membrane constituent lipid molecules by phospholipases, which are influenced by the physical state of cellular membranes. We discuss the possible involvement of nonbilayer forming lipids in the control of signaling mechanisms leading to TNF cytotoxicity.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - Dec 18 1997|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology