Highly increased TNF-sensitivity of tumor cells expressing a yeast delta-9-desaturase enzyme

E. Duda, Z. Uyorry, B. Maresca, L. Viyh

Research output: Contribution to journalArticle

Abstract

Tumor necrosis factor alpha (TNF) sensitivity of t'.nior cells is dramatically increased above 37 C It could be the result of a revive shortage of protective proteins and or altered physical properties of the biological membranes. We have cloned the della-Q-desaturase of Saccharomces cerevisiae into mammalian expression vectors and transformed differ, n, tumor cell lines Presence and expression of the yeast gene in the cells was proved bv PCR and RT-PCR Stable tmnsfonnam clones constitutivelv exposing the yeast gene show an increased ratio of monounsaturated fatty acids their (phospho)hpids. increased membranetlmditv and altered niorpholouy ilie cell clones grow more efficiently at suboptimal temperatures, however, highlv expressing clones can not survive at temperatures over 37 C TV transformant clones exhibit several hundred fold increased scnsitivitv to TJF, and can be killed by TNF concentrations ucnnally found in serum sample INF singallmg includes receptor clustering, a process expected to be influenced by membrane viscositv and in the signalling pathway at least four enzymes (phospholipases and sphingomyelmases) participate, that liberate consituents of the phospholipid bilaver Tlie activity of these enzymes is known to be dependent on the transition temperature of the membrane bilayer.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997

Fingerprint

Stearoyl-CoA Desaturase
Yeast
tumor necrosis factor-alpha
Tumors
Clone cells
Clone Cells
Tumor Necrosis Factor-alpha
Genes
Yeasts
Cells
clones
yeasts
Membranes
Biological membranes
Monounsaturated Fatty Acids
Phospholipases
Enzymes
enzymes
Neoplasms
Phospholipids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Highly increased TNF-sensitivity of tumor cells expressing a yeast delta-9-desaturase enzyme. / Duda, E.; Uyorry, Z.; Maresca, B.; Viyh, L.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

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