Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid

Ixchelt Cuaranta-Monroy, Zoltan Simandi, Zsuzsanna Kolostyak, Quang Minh Doan-Xuan, Szilard Poliska, Attila Horvath, Gergely Nagy, Zsolt Bacso, Laszlo Nagy

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21 Citations (Scopus)

Abstract

Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes mimic the gene-expression profile of subcutaneous isolated adipocytes in vivo remarkably well, much closer than 3T3-L1 derived ones. Moreover, the differentiated cells are in a monolayer, allowing a broad range of genome-wide studies in early and late stages of adipocyte differentiation to be performed.

Original languageEnglish
Pages (from-to)88-97
Number of pages10
JournalStem Cell Research
Volume13
Issue number1
DOIs
Publication statusPublished - Jul 2014

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ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Cuaranta-Monroy, I., Simandi, Z., Kolostyak, Z., Doan-Xuan, Q. M., Poliska, S., Horvath, A., Nagy, G., Bacso, Z., & Nagy, L. (2014). Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid. Stem Cell Research, 13(1), 88-97. https://doi.org/10.1016/j.scr.2014.04.015