High‐affinity binding of the basement membrane protein collagen type IV to the crystalline virulence surface protein array of Aeromonas salmonicida

Trevor J. Trust, Magdalena Kostrzynska, Levente Emödy, Torkel Wadström

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The surface of the fish pathogen Aeromonas salmonicida is covered by a paracrystalline array (the A‐layer) which is a virulence factor for the organism. Quantification of the ability of A. salmonicida cells to bind collagen types I and IV in a 125I‐radiolabelled liquid‐phase assay showed that A‐layer‐positive cells bound high levels of collagen type IV, but significantly lower levels of collagen type I. Collagen type IV binding was confirmed using non‐radiolabelled enzyme‐linked immunosorbent assays. 125I‐Collagen type IV binding was rapid, specific, saturable, high affinity, and essentially irreversible by unlabelled collagen type IV. The A‐layer was responsible for collagen type IV binding because binding was inactivated by selective removal of the A‐layer at pH 2.2, and neither isogenic A‐layer‐deficient A. salmonicida mutants nor strains of Aeromonas hydrophila possessing a morphologically similar paracrystalline array bound this basement membrane protein.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalMolecular Microbiology
Volume7
Issue number4
DOIs
Publication statusPublished - Feb 1993

    Fingerprint

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this