High-throughput screening of saliva for early detection of oral cancer

A pilot study

I. Szanto, L. Márk, A. Bona, G. Maasz, B. Sandor, G. Gelencser, Z. Turi, F. Gallyas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The success of tumour therapy depends considerably on early diagnosis. Therefore, we aimed to develop a widely available, cheap, non-invasive, high-throughput method suitable for screening high-risk populations, at least, for early signs of malignant transformation in the oral cavity. First, in order to identify suitable tumour marker candidates, we compared the protein patterns of five selected saliva samples obtained from healthy controls and tumour patients after electrophoretic separation, excised the bands that were consistently up-regulated in the tumour patients only, and performed matrix-assisted laser-desorption ionisation (MALDI)-time of flight (TOF) tandem mass spectrometry (MS/MS) analysis of the proteins in these bands after in-gel tryptic digestion. From the panel of proteins identified, we chose annexin 1 and peroxiredoxin 2 for further studies based on their presence in the saliva of all five oral cancer patients only. Then, we performed a homology search of protein databases using the primary sequence of each in silico tryptic fragment peptide of these two proteins as bait, and selected a unique peptide for each. Finally, we performed targeted MALDI-TOF MS peptide analysis in a blinded fashion on all samples obtained from 20 healthy controls and 22 tumour patients for the presence of these peptides. We found both peptides present in the saliva samples of all cancer patients only. Even though these tumour markers should be validated in a wider population, our results indicate that targeted MALDI-TOF MS analysis of unique peptides of putative saliva protein tumour biomarkers could be the method of choice for cost-efficient, high-throughput screening for the early detection of oral cancer.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalTechnology in Cancer Research and Treatment
Volume11
Issue number2
Publication statusPublished - Apr 2012

Fingerprint

Mouth Neoplasms
Early Detection of Cancer
Saliva
Tumor Biomarkers
Peptides
Lasers
Neoplasms
Proteins
High-Throughput Screening Assays
Peroxiredoxins
Annexins
Protein Databases
Peptide Fragments
Tandem Mass Spectrometry
Computer Simulation
Mouth
Early Diagnosis
Digestion
Gels
Costs and Cost Analysis

Keywords

  • Annexin 1
  • Early diagnosis
  • High-throughput analysis
  • MALDI-TOF MS
  • Oral cancer
  • Peroxiredoxin 2
  • Saliva proteomics
  • Targeted peptide analysis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

High-throughput screening of saliva for early detection of oral cancer : A pilot study. / Szanto, I.; Márk, L.; Bona, A.; Maasz, G.; Sandor, B.; Gelencser, G.; Turi, Z.; Gallyas, F.

In: Technology in Cancer Research and Treatment, Vol. 11, No. 2, 04.2012, p. 181-188.

Research output: Contribution to journalArticle

Szanto, I, Márk, L, Bona, A, Maasz, G, Sandor, B, Gelencser, G, Turi, Z & Gallyas, F 2012, 'High-throughput screening of saliva for early detection of oral cancer: A pilot study', Technology in Cancer Research and Treatment, vol. 11, no. 2, pp. 181-188.
Szanto, I. ; Márk, L. ; Bona, A. ; Maasz, G. ; Sandor, B. ; Gelencser, G. ; Turi, Z. ; Gallyas, F. / High-throughput screening of saliva for early detection of oral cancer : A pilot study. In: Technology in Cancer Research and Treatment. 2012 ; Vol. 11, No. 2. pp. 181-188.
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