High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems.

Dieter Vanderschaeghe, Andras Guttman, Nico Callewaert

Research output: Contribution to journalArticle

Abstract

Glycosylation research has gained significant attention in several research fields including immunology, protein production, and biomarker discovery. However, complex and time-consuming protocols are often necessary to obtain suitable samples for analysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans from serum samples. It only involves the subsequent addition of reagents and incubation in a PCR thermocycler. Moreover, this assay allows the detection of these glycans, which are only present in minute amounts in serum, on high-throughput microfluidics CE platforms including the MCE-202 MultiNA, 2100 Bioanalyzer, and eGene system. Using this clinical glycomics assay, we could reliably measure GlycoHepatoTest, a panel of biomarkers allowing the follow-up of chronic liver disease patients from the early stage onward.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume919
Publication statusPublished - 2013
Externally publishedYes

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Microfluidics
Capillary Electrophoresis
Polysaccharides
Biomarkers
Glycomics
Allergy and Immunology
Serum
Glycosylation
Research
Liver Diseases
Chronic Disease
Polymerase Chain Reaction
Proteins
8-aminopyrene-1,3,6-trisulfonic acid

ASJC Scopus subject areas

  • Medicine(all)

Cite this

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems. / Vanderschaeghe, Dieter; Guttman, Andras; Callewaert, Nico.

In: Methods in molecular biology (Clifton, N.J.), Vol. 919, 2013, p. 87-96.

Research output: Contribution to journalArticle

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