High-throughput genotyping by microchip electrophoresis

A. Guttman, Julia Khandurina, Z. Rónai, M. Sasvári

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Easy applicability of modern microfabrication technology to electrophoresis microchips has initiated a rapidly moving interdisciplinary field in analytical chemistry. Electric field-mediated separations in microfabricated devices are significantly faster than conventional electrophoresis methods and are usually completed in seconds to minutes. The flexibility of fluidic manipulations in electrophoresis microchips allows the use of a variety of separation techniques and conditions. In this study, large-scale genotyping of the repeat polymorphism in the regulatory (promoter) region of the serotonin transporter gene 5-HTT linked polymorphic region (5-HTTLPR) was attempted using polymerase chain reaction (PCR) amplification followed by rapid microchip electrophoresis analysis of the amplicons.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalJournal of Capillary Electrophoresis and Microchip Technology
Volume8
Issue number3-4
Publication statusPublished - 2003

Fingerprint

Microchip Electrophoresis
Electrophoresis
Throughput
Microchip Analytical Procedures
Microtechnology
Serotonin Plasma Membrane Transport Proteins
Nucleic Acid Regulatory Sequences
Genetic Promoter Regions
Microfabrication
Polymerase chain reaction
Fluidics
Polymorphism
Technology
Amplification
Equipment and Supplies
Polymerase Chain Reaction
Genes
Electric fields
Chemical analysis

Keywords

  • Genotyping
  • Microchip electrophoresis
  • Microfabricated devices
  • Repeat polymorphism
  • Serotonin transporter

ASJC Scopus subject areas

  • Electrochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

High-throughput genotyping by microchip electrophoresis. / Guttman, A.; Khandurina, Julia; Rónai, Z.; Sasvári, M.

In: Journal of Capillary Electrophoresis and Microchip Technology, Vol. 8, No. 3-4, 2003, p. 77-80.

Research output: Contribution to journalArticle

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