High sensitivity to autoxidation in neonatal calf erythrocytes

Possible mechanism of accelerated cell aging

S. Imre, M. Csornai, M. Balázs

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The suspension viscosity, formation of methaemoglobin and production of malondialdehyde (MDA) associated with the non-enzymatic oxidation of polyunsaturated fatty acids during auto-oxidation conditions in vitro have been compared in erythrocytes from young calves (2, 4 and 6 weeks of age) and mature cattle. The autoxidation conditions were designed to simulate the oxidative stress to which neonatal erythrocytes are exposed in vivo. Characterisation of lipid peroxidation was also undertaken by a combination of lipid fluorescent measurements and quantification of the superoxide dismutase (SOD) activities of the erythrocytes. The results demonstrated that high SOD activities in the erythrocytes of the neonatal calf was insufficient to afford protection against the increased autoxidation of haemoglobin and subsequent accumulation of lipid peroxidation products. High levels of methaemoglobin formation and lipid peroxidation were able to provide an explanation for an observed reduction in rheological adaptability (increased suspension viscosity) and an accelerated aging of the neonatal cells under in vivo conditions.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalMechanisms of Ageing and Development
Volume122
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Cell Aging
Aging of materials
Erythrocytes
Lipid Peroxidation
Lipids
Methemoglobin
Viscosity
Superoxide Dismutase
Suspensions
Oxidation
Oxidative stress
Malondialdehyde
Unsaturated Fatty Acids
Hemoglobins
Oxidative Stress

Keywords

  • Autoxidation
  • Calf erythrocytes
  • Lipid peroxidation
  • Rheology
  • Superoxide dismutase

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

High sensitivity to autoxidation in neonatal calf erythrocytes : Possible mechanism of accelerated cell aging. / Imre, S.; Csornai, M.; Balázs, M.

In: Mechanisms of Ageing and Development, Vol. 122, No. 1, 2001, p. 69-76.

Research output: Contribution to journalArticle

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