High resolution molecular cytogenetic techniques in plants

Pachytene-and fibre-FISH

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) is the most versatile and accurate molecular cytogenetic technique for determining euchromatic- heterochromatic boundaries and the locations of repetitive and single-copy DNA sequences and of chromosome-specific BAC clones on chromosomes. The combination of cytogenetic and genetic methods yields a highresolution physical map. FISH allows direct mapping of specific DNA sequences inside the cell (interphase nuclei), along meiotic pachytene chromosomes and isolated chromatin (DNA fibres). The increased sensitivity of the technique and its ability to detect gene locations provide a powerful research tool for genetic and pre-breeding studies. FISH-based physical mapping plays an important role and is increasingly used for studies at the cytological level on the chromatin organization that controls gene expression and regulation. The present minireview describes some of the benefits of alternative FISH-based techniques and their application for studying plant chromosomes and genomes.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalActa Agronomica Hungarica
Volume60
Issue number2
DOIs
Publication statusPublished - Jun 1 2012

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pachytene stage
fluorescence in situ hybridization
cytogenetics
chromosomes
physical chromosome mapping
chromatin
nucleotide sequences
methodology
interphase
clones
gene expression
genome
breeding
DNA
genes
cells

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

High resolution molecular cytogenetic techniques in plants : Pachytene-and fibre-FISH. / Linc, G.; Molnár-Láng, M.

In: Acta Agronomica Hungarica, Vol. 60, No. 2, 01.06.2012, p. 157-165.

Research output: Contribution to journalArticle

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