High-Resolution Adhesion Kinetics of EGCG-Exposed Tumor Cells on Biomimetic Interfaces: Comparative Monitoring of Cell Viability Using Label-Free Biosensor and Classic End-Point Assays

Beatrix Peter, Rita Ungai-Salanki, B. Szabó, Agoston G. Nagy, Inna Szekacs, Sz. Bősze, R. Horváth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A high-throughput label-free resonant waveguide grating biosensor, the Epic BenchTop, was utilized to in situ monitor the adhesion process of cancer cells on Arg-Gly-Asp tripeptide displaying biomimetic polymer surfaces. Using highly adherent human cervical adenocarcinoma (HeLa) cells as a model system, cell adhesion kinetic data with outstanding temporal resolution were obtained. We found that pre-exposing the cells to various concentrations of the main extract of green tea, the (-)-epigallocatechin gallate (EGCG), largely affected the temporal evolution of the adhesion process. For unexposed and low dosed cells, sigmoid shaped spreading kinetics was recorded. Higher dose of EGCG resulted in a complete absence of the sigmoidal character, and displayed adsorption-like kinetics. By using the first derivatives of the kinetic curves, a simple model was developed to quantify the sigmoidal character and the transition from sigmoidal to adsorption-like kinetics. The calculations showed that the transition happened at EGCG concentration of around 60 μg/mL. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide end-point assay, we concluded that EGCG is cytostatic but not cytotoxic. The effect of EGCG was also characterized by flow cytometry. We concluded that, using the introduced label-free methodology, the shape of the cell adhesion kinetic curves can be used to quantify in vitro cell viability in a fast, cost-effective, and highly sensitive manner.

Original languageEnglish
Pages (from-to)3882-3891
Number of pages10
JournalACS Omega
Volume3
Issue number4
DOIs
Publication statusPublished - Jan 1 2018

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Biomimetics
Biosensors
Labels
Tumors
Assays
Adhesion
Cells
Kinetics
Monitoring
Cell adhesion
Adsorption
Flow cytometry
Cytostatic Agents
epigallocatechin gallate
Polymers
Waveguides
Throughput
Derivatives
Costs

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

High-Resolution Adhesion Kinetics of EGCG-Exposed Tumor Cells on Biomimetic Interfaces : Comparative Monitoring of Cell Viability Using Label-Free Biosensor and Classic End-Point Assays. / Peter, Beatrix; Ungai-Salanki, Rita; Szabó, B.; Nagy, Agoston G.; Szekacs, Inna; Bősze, Sz.; Horváth, R.

In: ACS Omega, Vol. 3, No. 4, 01.01.2018, p. 3882-3891.

Research output: Contribution to journalArticle

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