High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia

Zsófia Balogh, Lilla Reiniger, Hajnalka Rajnai, Judit Csomor, Ágota Szepesi, Anikó Balogh, Linda Deák, Éva Gagyi, Csaba Bödör, A. Matolcsy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In lymph nodes, chronic lymphocytic leukemia (CLL) cells (prolymphocytes and paraimmunoblasts) form proliferation centers (PCs), which are also known as pseudofollicles. To reveal whether PCs play a role in the accumulation of genetic alterations in CLL, we compared deletion at 11q22.3, 13q14.3, and 17p13.1 loci and trisomy 12 by the fluorescence in situ hybridization (FISH) technique in PCs versus surrounding small lymphocytes (SLs) in 12 formalin-fixed paraffin-embedded (FFPE) lymph nodes. The FFPE sections were stained with methylene blue and PCs were marked by laser beam. Subsequent FISH analysis was performed, relocalizing the previously defined regions. Loss of 11q was detected in five cases, loss of 13q in two cases, loss of 17p in two cases, and trisomy 12 in one case. In seven cases PCs contained a significantly higher ratio of cells with genetic alterations compared with the surrounding SLs. Our results show that CLL cells with genetic alterations tend to accumulate in PCs. The clonal expansion of the cell population carrying genetic alterations within PCs may contribute to CLL progression.

Original languageEnglish
Pages (from-to)1080-1084
Number of pages5
JournalLeukemia and Lymphoma
Volume52
Issue number6
DOIs
Publication statusPublished - Jun 2011

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Trisomy
Fluorescence In Situ Hybridization
Paraffin
Formaldehyde
Lymph Nodes
Lymphocytes
Methylene Blue
Population Genetics
Lasers

Keywords

  • chronic lymphocytic leukemia
  • genome instability
  • Proliferation center
  • pseudofollicle

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia. / Balogh, Zsófia; Reiniger, Lilla; Rajnai, Hajnalka; Csomor, Judit; Szepesi, Ágota; Balogh, Anikó; Deák, Linda; Gagyi, Éva; Bödör, Csaba; Matolcsy, A.

In: Leukemia and Lymphoma, Vol. 52, No. 6, 06.2011, p. 1080-1084.

Research output: Contribution to journalArticle

Balogh, Z, Reiniger, L, Rajnai, H, Csomor, J, Szepesi, Á, Balogh, A, Deák, L, Gagyi, É, Bödör, C & Matolcsy, A 2011, 'High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia', Leukemia and Lymphoma, vol. 52, no. 6, pp. 1080-1084. https://doi.org/10.3109/10428194.2011.555889
Balogh, Zsófia ; Reiniger, Lilla ; Rajnai, Hajnalka ; Csomor, Judit ; Szepesi, Ágota ; Balogh, Anikó ; Deák, Linda ; Gagyi, Éva ; Bödör, Csaba ; Matolcsy, A. / High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia. In: Leukemia and Lymphoma. 2011 ; Vol. 52, No. 6. pp. 1080-1084.
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