High pericardial fluid levels of endothelin are not caused by altered neutral endopeptidase activity in cardiac patients

P. Turbucz, P. Kiss, F. Horkay, I. Szokodi, R. DeChâtel, L. Selmeci, A. Juhász-Nagy, I. Karádi, M. Tóth

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Abstract

We have previously detected in cardiac patients severalfold higher levels of endothelin (ET) in the pericardial fluid (PF) than in the plasma (PL). We postulated that this is due to different activities of neutral endopeptidase (NEP) in the two compartments. With approval of the ethical committee and informed consent by 32 patients (18 men, 14 women, aged 62 ± 2 years; NYHA II-IV), PF was taken during cardiac surgery. PL samples were obtained on the day of surgery before premedication. ET was measured by radioimmunoassay after extraction (SepPakC18). NEP activity was measured by a microplate-based kinetic enzyme assay over 120 min. PF ET (78 ± 11 pg/ml) was significantly (p <0.05) higher than PL ET (3.38 ± 0.48 pg/ml). The PF/PL ratio was 38 ± 14, range 7-200. PF ET was inversely related to the NYHA state of the patients, whereas a similar relation was not found with PL ET. PL and PF ET levels did not correlate. In HPLC, the total immunoreactive ET activity co-eluted with the human ET standard. PF NEP activity (2.26 ± 0.12 U/l) was lower (p <0.05) than PL NEP (3.62 ± 0.22 U/I). PL NEP was not different from that of healthy controls (3.28 ± 0.22 U/L; n = 50). No correlation was found between NEP in either compartment and the NYHA state of the patients. ET concentration and NEP activity did not correlate in PF or PL. We conclude that ET is extremely high in the PF of cardiac patients and that this is not caused by altered NEP activity.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume31
Issue numberSUPPL. 1
Publication statusPublished - 1998

Fingerprint

Neprilysin
Endothelins
Pericardial Fluid
Premedication
Enzyme Assays
Informed Consent
Ambulatory Surgical Procedures
Thoracic Surgery
Radioimmunoassay
High Pressure Liquid Chromatography

Keywords

  • Cardiac patients
  • Endothelin
  • Neutral endopeptidase activity
  • Pericardial fluid

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

@article{dcf9d7c1746d417aa89f7519921f749e,
title = "High pericardial fluid levels of endothelin are not caused by altered neutral endopeptidase activity in cardiac patients",
abstract = "We have previously detected in cardiac patients severalfold higher levels of endothelin (ET) in the pericardial fluid (PF) than in the plasma (PL). We postulated that this is due to different activities of neutral endopeptidase (NEP) in the two compartments. With approval of the ethical committee and informed consent by 32 patients (18 men, 14 women, aged 62 ± 2 years; NYHA II-IV), PF was taken during cardiac surgery. PL samples were obtained on the day of surgery before premedication. ET was measured by radioimmunoassay after extraction (SepPakC18). NEP activity was measured by a microplate-based kinetic enzyme assay over 120 min. PF ET (78 ± 11 pg/ml) was significantly (p <0.05) higher than PL ET (3.38 ± 0.48 pg/ml). The PF/PL ratio was 38 ± 14, range 7-200. PF ET was inversely related to the NYHA state of the patients, whereas a similar relation was not found with PL ET. PL and PF ET levels did not correlate. In HPLC, the total immunoreactive ET activity co-eluted with the human ET standard. PF NEP activity (2.26 ± 0.12 U/l) was lower (p <0.05) than PL NEP (3.62 ± 0.22 U/I). PL NEP was not different from that of healthy controls (3.28 ± 0.22 U/L; n = 50). No correlation was found between NEP in either compartment and the NYHA state of the patients. ET concentration and NEP activity did not correlate in PF or PL. We conclude that ET is extremely high in the PF of cardiac patients and that this is not caused by altered NEP activity.",
keywords = "Cardiac patients, Endothelin, Neutral endopeptidase activity, Pericardial fluid",
author = "P. Turbucz and P. Kiss and F. Horkay and I. Szokodi and R. DeCh{\^a}tel and L. Selmeci and A. Juh{\'a}sz-Nagy and I. Kar{\'a}di and M. T{\'o}th",
year = "1998",
language = "English",
volume = "31",
journal = "Journal of Cardiovascular Pharmacology",
issn = "0160-2446",
publisher = "Lippincott Williams and Wilkins",
number = "SUPPL. 1",

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T1 - High pericardial fluid levels of endothelin are not caused by altered neutral endopeptidase activity in cardiac patients

AU - Turbucz, P.

AU - Kiss, P.

AU - Horkay, F.

AU - Szokodi, I.

AU - DeChâtel, R.

AU - Selmeci, L.

AU - Juhász-Nagy, A.

AU - Karádi, I.

AU - Tóth, M.

PY - 1998

Y1 - 1998

N2 - We have previously detected in cardiac patients severalfold higher levels of endothelin (ET) in the pericardial fluid (PF) than in the plasma (PL). We postulated that this is due to different activities of neutral endopeptidase (NEP) in the two compartments. With approval of the ethical committee and informed consent by 32 patients (18 men, 14 women, aged 62 ± 2 years; NYHA II-IV), PF was taken during cardiac surgery. PL samples were obtained on the day of surgery before premedication. ET was measured by radioimmunoassay after extraction (SepPakC18). NEP activity was measured by a microplate-based kinetic enzyme assay over 120 min. PF ET (78 ± 11 pg/ml) was significantly (p <0.05) higher than PL ET (3.38 ± 0.48 pg/ml). The PF/PL ratio was 38 ± 14, range 7-200. PF ET was inversely related to the NYHA state of the patients, whereas a similar relation was not found with PL ET. PL and PF ET levels did not correlate. In HPLC, the total immunoreactive ET activity co-eluted with the human ET standard. PF NEP activity (2.26 ± 0.12 U/l) was lower (p <0.05) than PL NEP (3.62 ± 0.22 U/I). PL NEP was not different from that of healthy controls (3.28 ± 0.22 U/L; n = 50). No correlation was found between NEP in either compartment and the NYHA state of the patients. ET concentration and NEP activity did not correlate in PF or PL. We conclude that ET is extremely high in the PF of cardiac patients and that this is not caused by altered NEP activity.

AB - We have previously detected in cardiac patients severalfold higher levels of endothelin (ET) in the pericardial fluid (PF) than in the plasma (PL). We postulated that this is due to different activities of neutral endopeptidase (NEP) in the two compartments. With approval of the ethical committee and informed consent by 32 patients (18 men, 14 women, aged 62 ± 2 years; NYHA II-IV), PF was taken during cardiac surgery. PL samples were obtained on the day of surgery before premedication. ET was measured by radioimmunoassay after extraction (SepPakC18). NEP activity was measured by a microplate-based kinetic enzyme assay over 120 min. PF ET (78 ± 11 pg/ml) was significantly (p <0.05) higher than PL ET (3.38 ± 0.48 pg/ml). The PF/PL ratio was 38 ± 14, range 7-200. PF ET was inversely related to the NYHA state of the patients, whereas a similar relation was not found with PL ET. PL and PF ET levels did not correlate. In HPLC, the total immunoreactive ET activity co-eluted with the human ET standard. PF NEP activity (2.26 ± 0.12 U/l) was lower (p <0.05) than PL NEP (3.62 ± 0.22 U/I). PL NEP was not different from that of healthy controls (3.28 ± 0.22 U/L; n = 50). No correlation was found between NEP in either compartment and the NYHA state of the patients. ET concentration and NEP activity did not correlate in PF or PL. We conclude that ET is extremely high in the PF of cardiac patients and that this is not caused by altered NEP activity.

KW - Cardiac patients

KW - Endothelin

KW - Neutral endopeptidase activity

KW - Pericardial fluid

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M3 - Article

VL - 31

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

SN - 0160-2446

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