High-performance ultrathin layer agarose gel electrophoresis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Large scale, high-resolution DNA fragment analysis, such as genotyping, mapping and genetic profiling requires an affordable, fully automated high-throughput gel electrophoresis based separation device that enables rapid, high-performance analysis in a wide molecular weight range. In this article a novel approach is described that greatly enhances the productivity of DNA fragment analysis by automating the current manual procedure and also reducing the separation time and human intervention from sample loading to data analysis. The ultrathin layer, multilane, high-performance agarose gel electrophoresis system employs integrated scanning laser induced fluorescence-avalanche photodiode detection and combines the advantages of conventional slab and capillary gel electrophoresis. The separation platform is fabricated in a way that the sieving matrix can be easily replaced in the separation cassette for each run. Visualization of the DNA fragments is accomplished by 'in migratio' complexation during the electrophoresis process with ultra-sensitive fluorescent agents, also enabling real-time imaging and data analysis. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)694-702
Number of pages9
JournalTrAC - Trends in Analytical Chemistry
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 1 1999

Keywords

  • DNA fragment analysis
  • Gel electrophoresis
  • Real-time imaging
  • Ultrathin layer agarose

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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