High-performance separation methods in the analysis of a new peptide family: The galanins

Tamás Janáky, Endre Szabó, Lajos Baláspiri, Basel Adi, Botond Penke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An analytical investigation of a new peptide family, the human galanins and their fragments, was carried out by reversed-phase HPLC, capillary zone electrophoresis (CZE) at different pH values and micellar electrokinetic capillary chromatography (MECC) in phosphate-borate-sodium dodecyl sulphate buffer. None of the methods seems to be superior to the others. The complementary nature of the electrophoretic methods is obvious when the profiles of peptides are compared; impurities not separated by HPLC are separated by CZE or MECC and vice versa. With these three different separation methods, a more complex analytical control of the synthetic work can be achieved.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume676
Issue number1
DOIs
Publication statusPublished - Feb 9 1996

Keywords

  • Galanins
  • Peptides

ASJC Scopus subject areas

  • Chemistry(all)

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